Cloned (Comment) | Organism |
---|---|
both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
butyryl-CoA | - |
Pseudomonas aeruginosa | |
CoASH | - |
Pseudomonas aeruginosa | |
N6'-acetyl kanamycin A | - |
Pseudomonas aeruginosa | |
paromomycin | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
isepamicin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.001 | - |
kanamycin A | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.001 | - |
amikacin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.001 | - |
netilmicin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.0012 | - |
neomycin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.003 | - |
dibekacin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.0083 | - |
gentamicin | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa | |
0.009 | - |
acetyl-CoA | reaction with AAC (6?)-Ib' domain | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
both acetyltransferase domains of the bifunctional enzyme AAC(3)-Ib/AAC(6')-Ib' are cloned and purified | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + amikacin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylamikacin | - |
? | |
acetyl-CoA + dibekacin | - |
Pseudomonas aeruginosa | CoA + N6'-acetyldibekacin | - |
? | |
acetyl-CoA + gentamicin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylgentamicin | - |
? | |
acetyl-CoA + isepamicin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylisepamicin | - |
? | |
acetyl-CoA + kanamycin A | - |
Pseudomonas aeruginosa | CoA + N6'-acetylkanamycin A | - |
? | |
acetyl-CoA + neomycin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylneomycin | - |
? | |
acetyl-CoA + netilmicin | - |
Pseudomonas aeruginosa | CoA + N6'-acetylnetilmicin | - |
? | |
additional information | no activity with fortimicin A. Bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b'?domain exhibits a broad substrate spectrum | Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAC(3)-Ib/AAC(6')-Ib' | bifunctional antibiotic-resistance enzyme. Both domains are acetyltransferases. The AAC(3)-Ib domain appears to be highly specific to fortimicin A and gentamicin as substrates, while the AAC(6')-b' domain (EC 2.3.1.82) exhibits a broad substrate spectrum | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
acetyl-CoA | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
1.3 | - |
netilmicin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
1.3 | - |
dibekacin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
2 | - |
gentamicin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
2.4 | - |
kanamycin A | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
2.4 | - |
isepamicin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
3.5 | - |
amikacin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa | |
6 | - |
neomycin | reaction with AAC (6')-Ib' domain | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
paromomycin | versus kanamycin A, K(is) | Pseudomonas aeruginosa | |
0.02 | - |
CoASH | versus kanamycin A. K(is) | Pseudomonas aeruginosa | |
0.03 | - |
CoASH | versus acetyl-CoA, K(is) | Pseudomonas aeruginosa | |
0.04 | - |
paromomycin | versus acetyl-CoA, K(ii) | Pseudomonas aeruginosa | |
0.04 | - |
6'-N-acetylated kanamycin A | versus acetyl-CoA. K(is) | Pseudomonas aeruginosa | |
0.05 | - |
6'-N-acetylated kanamycin A | versus kanamycin A. K(is) | Pseudomonas aeruginosa | |
0.11 | - |
butyryl-CoA | versus acetyl-CoA, K(is) | Pseudomonas aeruginosa | |
0.15 | - |
butyryl-CoA | versus kanamycin A, K(is) | Pseudomonas aeruginosa | |
0.2 | - |
CoASH | versus kanamycin A. K(ii) | Pseudomonas aeruginosa | |
0.38 | - |
6'-N-acetylated kanamycin A | versus acetyl-CoA. K(ii) | Pseudomonas aeruginosa | |
0.55 | - |
6'-N-acetylated kanamycin A | versus kanamycin A. K(ii) | Pseudomonas aeruginosa | |
0.57 | - |
butyryl-CoA | versus kanamycin A, K(ii) | Pseudomonas aeruginosa |