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Literature summary for 2.3.1.82 extracted from

  • Boehr, D.D.; Jenkins, S.I.; Wright, G.D.
    The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6'-aminoglycoside phosphotransferase-2". The role of Asp-99 as an active site base important for acetyl transfer (2003), J. Biol. Chem., 278, 12873-12880.
    View publication on PubMed
Search for more literature for 2.3.1.82

Protein Variants

Protein Variants Comment Organism
D99A 39fold decrease in the ratio of turnover number to Km-value for acetyl-CoA, 215fold decrease in the ratio of turnover number to Km-value for kanamycin A, 86fold decrease in the ratio of turnover number to Km-value for neamine Enterococcus sp.
D99E 4.7fold decrease in the ratio of turnover number to Km-value for acetyl-CoA, 31fold decrease in the ratio of turnover number to Km-value for kanamycin A, 19.3fold decrease in the ratio of turnover number to Km-value for neamine, 134fold decrease in the ratio of turnover number to Km-value for fortimicin A Enterococcus sp.
D99N 7fold decrease in the ratio of turnover number to Km-value for acetyl-CoA, 130fold decrease in the ratio of turnover number to Km-value for kanamycin A, 102fold decrease in the ratio of turnover number to Km-value for neamine Enterococcus sp.
Y96F minor effects on the steady state kinetic parameters, largest effects for paromomycin, 5.5fold decrease in the ratio kcat /Km compared with wild-type Enterococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
1-(bromomethyl)phenanthrene inactivates through covalent modification of Asp99 Enterococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.0254
-
 fortimicin A pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.031
-
 kanamycin A pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.038
-
 acetyl-CoA pH 7.5, 37°C,wild-type AAC(6')-Ie Enterococcus sp.
0.0399
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.0435
-
 fortimicin A pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
0.0453
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.07
-
 fortimicin A pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.0736
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
0.078
-
 neamine pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.079
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
0.0902
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.102
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.229
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.324
-
 paromomycin pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.326
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
0.443
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.509
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.771
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.771
-
 paromomycin pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.

Organism

Organism UniProt Comment Textmining
Enterococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + fortimicin A
-
 Enterococcus sp. CoA + N6'-acetylfortimicin A
-
 ?
acetyl-CoA + kanamycin A
-
 Enterococcus sp. CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + neamine
-
 Enterococcus sp. CoA + N6'-acetylneamine
-
 ?
acetyl-CoA + paromomycin
-
 Enterococcus sp. CoA + O6'-acetylparomomycin
-
 ?
additional information Asp-88 acts as an active site base in the molecular mechanism of AAC(6')-Ie Enterococcus sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
AAC(6')-Ie aminoglycoside acetyltransferase domain of acetyltransferase-6'-aminoglycoside phosphotransferase-2" Enterococcus sp.
acetyltransferase-6'-aminoglycoside phosphotransferase-2"
-
 Enterococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0002
-
 fortimicin A pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.0004
-
 paromomycin pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.0008
-
 fortimicin A pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.006
-
 fortimicin A pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.026
-
 paromomycin pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
0.028
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.029
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.033
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.035
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie D99A Enterococcus sp.
0.038
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.059
-
 paromomycin pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.066
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.11
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.17
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie D99N Enterococcus sp.
0.19
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie D99E Enterococcus sp.
0.28
-
 fortimicin A pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.43
-
 neamine pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
0.45
-
 fortimicin A pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
1.1
-
 kanamycin A pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
1.2
-
 acetyl-CoA pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.
1.3
-
 acetyl-CoA pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
1.3
-
 neamine pH 7.5, 37°C, mutant AAC(6')-Ie Y96F Enterococcus sp.
1.7
-
 kanamycin A pH 7.5, 37°C, wild-type AAC(6')-Ie Enterococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 wild-type AAC(6')-Ie with acetyl-CoA or neamine as variable substrate Enterococcus sp.