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Literature summary for 2.3.1.82 extracted from

  • Wright, G.D.; Ladak, P.
    Overexpression and characterization of the chromosomal aminoglycoside 6'-N-acetyltransferase from Enterococcus faecium (1997), Antimicrob. Agents Chemother., 41, 956-960.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.82

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecium

Protein Variants

Protein Variants Comment Organism
F171L
-
 Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
butyryl-CoA
-
 Enterococcus faecium
dibekacin
-
 Enterococcus faecium
kanamycin A
-
 Enterococcus faecium
kanamycin B
-
 Enterococcus faecium
netilmicin
-
 Enterococcus faecium
sisomycin
-
 Enterococcus faecium
tobramycin
-
 Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00531
-
 neomycin C pH 6.0, 37°C Enterococcus faecium
0.00582
-
 neamine pH 6.0, 37°C Enterococcus faecium
0.00638
-
 netilmicin pH 6.0, 37°C Enterococcus faecium
0.00908
-
 ribostamycin pH 6.0, 37°C Enterococcus faecium
0.0117
-
 sisomicin pH 6.0, 37°C Enterococcus faecium
0.0131
-
 amikacin pH 6.0, 37°C Enterococcus faecium
0.014
-
 butirosin pH 6.0, 37°C Enterococcus faecium
0.0189
-
 kanamycin B pH 6.0, 37°C Enterococcus faecium
0.0195
-
 propionyl-CoA pH 6.0, 37°C Enterococcus faecium
0.0199
-
 kanamycin A pH 6.0, 37°C Enterococcus faecium
0.022
-
 tobramycin pH 6.0, 37°C Enterococcus faecium
0.0223
-
 gentamicin B pH 6.0, 37°C Enterococcus faecium
0.0235
-
 acetyl-CoA pH 6.0, 37°C Enterococcus faecium
0.036
-
 dibekacin pH 6.0, 37°C Enterococcus faecium

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Enterococcus faecium involved in resistance to antibiotics ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + amikacin
-
 Enterococcus faecium CoA + N6'-acetylamikacin
-
 ?
acetyl-CoA + butirosin
-
 Enterococcus faecium CoA + N6'-acetylbutirosin
-
 ?
acetyl-CoA + dibekacin
-
 Enterococcus faecium CoA + N6'-acetyldibekacin
-
 ?
acetyl-CoA + gentamicin B
-
 Enterococcus faecium CoA + N6'-acetylgentamicin B
-
 ?
acetyl-CoA + kanamycin A
-
 Enterococcus faecium CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + kanamycin B
-
 Enterococcus faecium CoA + N6'-acetylkanamycin B
-
 ?
acetyl-CoA + neamine
-
 Enterococcus faecium CoA + 6'-N-acetylneamine
-
 ?
acetyl-CoA + neomycin C
-
 Enterococcus faecium CoA + N6'-acetylneomycin C
-
 ?
acetyl-CoA + netilmicin
-
 Enterococcus faecium CoA + N6'-acetylnetilmicin
-
 ?
acetyl-CoA + ribostamycin
-
 Enterococcus faecium CoA + N6'-acetylribostamycin
-
 ?
acetyl-CoA + sisomicin
-
 Enterococcus faecium CoA + N6'-acetylsisomicin
-
 ?
acetyl-CoA + tobramycin
-
 Enterococcus faecium CoA + N6'-acetyltobramycin
-
 ?
additional information acetyl-CoA and propionyl-CoA are comparable substrates, but butyryl-CoA is not Enterococcus faecium ?
-
 ?
additional information involved in resistance to antibiotics Enterococcus faecium ?
-
 ?
propionyl-CoA + kanamycin A
-
 Enterococcus faecium CoA + N6'-propionylkanamycin A
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.106
-
 amikacin pH 6.0, 37°C Enterococcus faecium
0.205
-
 neomycin C pH 6.0, 37°C Enterococcus faecium
0.317
-
 propionyl-CoA pH 6.0, 37°C Enterococcus faecium
0.338
-
 ribostamycin pH 6.0, 37°C Enterococcus faecium
0.37
-
 sisomicin pH 6.0, 37°C Enterococcus faecium
0.388
-
 gentamicin B pH 6.0, 37°C Enterococcus faecium
0.403
-
 acetyl-CoA pH 6.0, 37°C Enterococcus faecium
0.419
-
 neamine pH 6.0, 37°C Enterococcus faecium
0.466
-
 butirosin pH 6.0, 37°C Enterococcus faecium
0.512
-
 netilmicin pH 6.0, 37°C Enterococcus faecium
0.632
-
 dibekacin pH 6.0, 37°C Enterococcus faecium
0.816
-
 kanamycin A pH 6.0, 37°C Enterococcus faecium
1.08
-
 kanamycin B pH 6.0, 37°C Enterococcus faecium
1.11
-
 tobramycin pH 6.0, 37°C Enterococcus faecium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.059
-
 tobramycin pH 6.0, 37°C Enterococcus faecium
0.063
-
 sisomicin pH 6.0, 37°C Enterococcus faecium
0.072
-
 dibekacin pH 6.0, 37°C Enterococcus faecium
0.117
-
 kanamycin B pH 6.0, 37°C Enterococcus faecium
0.142
-
 netilmicin pH 6.0, 37°C Enterococcus faecium
0.196
-
 kanamycin A pH 6.0, 37°C Enterococcus faecium