General Stability | Organism |
---|---|
dialysis in the absence of Mg2+ causes irreversible loss of activity | Escherichia coli |
Mg2+ stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gentamicin A | - |
Escherichia coli | |
gentamine A | - |
Escherichia coli | |
N6'-Acetylgentamicin C1a | - |
Escherichia coli | |
N6'-acetylneomycin B | - |
Escherichia coli | |
paromamine | - |
Escherichia coli | |
paromomycin | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | constitutive enzyme | ? | - |
? | |
additional information | Escherichia coli | involved in resistance to antibiotics | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains W677 carrying either R factor R-5 or R-79 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, several months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + gentamicin C1a | - |
Escherichia coli | CoA + N6'-acetylgentamicin C1a | - |
? | |
acetyl-CoA + gentamicin C2 | poor substrate, not gentamicin A and C1 | Escherichia coli | CoA + N6'-acetylgentgamicin C2 | - |
? | |
acetyl-CoA + hybrimycin A1 | - |
Escherichia coli | CoA + N6'-acetylhybrimycin A1 | - |
? | |
acetyl-CoA + hybrimycin A2 | - |
Escherichia coli | CoA + N6'-acetylhybrimycin A2 | - |
? | |
acetyl-CoA + hybrimycin A3 | - |
Escherichia coli | CoA + N6'-acetylhybrimycin A3 | - |
? | |
acetyl-CoA + hybrimycin B1 | - |
Escherichia coli | CoA + N6'-acetylhybrimycin B1 | - |
? | |
acetyl-CoA + hybrimycin B2 | - |
Escherichia coli | CoA + N6'-acetylhybrimycin B2 | - |
? | |
acetyl-CoA + hybrimycin B3 | - |
Escherichia coli | CoA + N6'-acetylhybrimycin B3 | - |
? | |
acetyl-CoA + kanamycin A | - |
Escherichia coli | CoA + N6'-acetylkanamycin A | - |
? | |
acetyl-CoA + kanamycin B | - |
Escherichia coli | CoA + N6'-acetylkanamycin B | - |
? | |
acetyl-CoA + neamine | - |
Escherichia coli | CoA + 6'-N-acetylneamine | - |
? | |
acetyl-CoA + nebramycin factor 4 | no activity with factor 2 | Escherichia coli | CoA + N6'-acetylnebramycin factor 4 | - |
? | |
acetyl-CoA + nebramycin factor 6 | no activity with factor 2 | Escherichia coli | CoA + N6'-acetylnebramycin factor 6 | - |
? | |
acetyl-CoA + neomycin B | - |
Escherichia coli | CoA + N6'-acetylneomycin B | - |
? | |
acetyl-CoA + neomycin C | - |
Escherichia coli | CoA + N6'-acetylneomycin C | - |
? | |
additional information | the smallest antibiotic moiety required for recognition as a substrate by the acetylating enzyme is a 6-amino-6-deoxy-hexose glycosidically linked to a streptamine or deoxystreptamine ring | Escherichia coli | ? | - |
? | |
additional information | constitutive enzyme | Escherichia coli | ? | - |
? | |
additional information | involved in resistance to antibiotics | Escherichia coli | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 35% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly | Escherichia coli |
42 | - |
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 70% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
reaction with kanamycin, neomycin, hybrimycin, nebramycin factor 4 and 6 | Escherichia coli |
7.6 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.3 | 7.6 | pH 5.3: about 50% of maximal activity, pH 7.6: about 20% of maximal activity, kanamycin A | Escherichia coli |