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Literature summary for 2.3.1.82 extracted from

  • Benveniste, R.; Davies, J.
    Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor (1971), Biochemistry, 10, 1787-1796.
    View publication on PubMed

General Stability

General Stability Organism
dialysis in the absence of Mg2+ causes irreversible loss of activity Escherichia coli
Mg2+ stabilizes Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
gentamicin A
-
Escherichia coli
gentamine A
-
Escherichia coli
N6'-Acetylgentamicin C1a
-
Escherichia coli
N6'-acetylneomycin B
-
Escherichia coli
paromamine
-
Escherichia coli
paromomycin
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli constitutive enzyme ?
-
?
additional information Escherichia coli involved in resistance to antibiotics ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
strains W677 carrying either R factor R-5 or R-79
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + gentamicin C1a
-
Escherichia coli CoA + N6'-acetylgentamicin C1a
-
?
acetyl-CoA + gentamicin C2 poor substrate, not gentamicin A and C1 Escherichia coli CoA + N6'-acetylgentgamicin C2
-
?
acetyl-CoA + hybrimycin A1
-
Escherichia coli CoA + N6'-acetylhybrimycin A1
-
?
acetyl-CoA + hybrimycin A2
-
Escherichia coli CoA + N6'-acetylhybrimycin A2
-
?
acetyl-CoA + hybrimycin A3
-
Escherichia coli CoA + N6'-acetylhybrimycin A3
-
?
acetyl-CoA + hybrimycin B1
-
Escherichia coli CoA + N6'-acetylhybrimycin B1
-
?
acetyl-CoA + hybrimycin B2
-
Escherichia coli CoA + N6'-acetylhybrimycin B2
-
?
acetyl-CoA + hybrimycin B3
-
Escherichia coli CoA + N6'-acetylhybrimycin B3
-
?
acetyl-CoA + kanamycin A
-
Escherichia coli CoA + N6'-acetylkanamycin A
-
?
acetyl-CoA + kanamycin B
-
Escherichia coli CoA + N6'-acetylkanamycin B
-
?
acetyl-CoA + neamine
-
Escherichia coli CoA + 6'-N-acetylneamine
-
?
acetyl-CoA + nebramycin factor 4 no activity with factor 2 Escherichia coli CoA + N6'-acetylnebramycin factor 4
-
?
acetyl-CoA + nebramycin factor 6 no activity with factor 2 Escherichia coli CoA + N6'-acetylnebramycin factor 6
-
?
acetyl-CoA + neomycin B
-
Escherichia coli CoA + N6'-acetylneomycin B
-
?
acetyl-CoA + neomycin C
-
Escherichia coli CoA + N6'-acetylneomycin C
-
?
additional information the smallest antibiotic moiety required for recognition as a substrate by the acetylating enzyme is a 6-amino-6-deoxy-hexose glycosidically linked to a streptamine or deoxystreptamine ring Escherichia coli ?
-
?
additional information constitutive enzyme Escherichia coli ?
-
?
additional information involved in resistance to antibiotics Escherichia coli ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 35% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly Escherichia coli
42
-
pH 7.4, 10 mM MgCl2, 0.6 mM 2-mercaptoethanol, about 70% loss of activity after 10 min, acetyl-CoA prevents, kanamycin protects slightly Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
reaction with kanamycin, neomycin, hybrimycin, nebramycin factor 4 and 6 Escherichia coli
7.6
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.3 7.6 pH 5.3: about 50% of maximal activity, pH 7.6: about 20% of maximal activity, kanamycin A Escherichia coli