Literature summary for 2.3.1.81 extracted from

Galimand, M.; Fishovitz, J.; Lambert, T.; Barbe, V.; Zajicek, J.; Mobashery, S.; Courvalin, P.
AAC(3)-XI, a new aminoglycoside 3-N-acetyltransferase from Corynebacterium striatum (2015), Antimicrob. Agents Chemother., 59, 5647-5653.

Cloned(Commentary)

Cloned (Comment)	Organism
gne aac(3)-XI, DNA and amino acid sequence determination and analysis, genomic organization, the aac(3)-XI gene is located downstream from a gene for a transposase (TnpCx) and upstream from that for a two-component system sensor kinase, the sequence upstream from aac(3)-XI does not reveal any putative promoter, recombinant expression of His-tagged enzyme in Escherichia coli strains TOP10 and BL21 Star(DE3)	Corynebacterium striatum

Cloned (Comment)

Organism

gne aac(3)-XI, DNA and amino acid sequence determination and analysis, genomic organization, the aac(3)-XI gene is located downstream from a gene for a transposase (TnpCx) and upstream from that for a two-component system sensor kinase, the sequence upstream from aac(3)-XI does not reveal any putative promoter, recombinant expression of His-tagged enzyme in Escherichia coli strains TOP10 and BL21 Star(DE3)

Corynebacterium striatum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	strain BM4687 is resistant to gentamicin and tobramycin but susceptible to kanamycin A and amikacin	Corynebacterium striatum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	Michaelis-Menten kinetics	Corynebacterium striatum
0.0027	-	acetyl-CoA	pH 6.0, 37°C	Corynebacterium striatum
0.0027	-	acetyl-CoA	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum
0.0092	-	dibekacin	pH 6.0, 37°C	Corynebacterium striatum
0.0092	-	dibekacin	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum
0.0138	-	kanamycin B	pH 6.0, 37°C	Corynebacterium striatum
0.0138	-	kanamycin B	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
acetyl-CoA + a 2-deoxystreptamine antibiotic	Corynebacterium striatum	-	CoA + N3-acetyl-2-deoxystreptamine antibiotic	-	?
acetyl-CoA + a 2-deoxystreptamine antibiotic	Corynebacterium striatum BM4687	-	CoA + N3-acetyl-2-deoxystreptamine antibiotic	-	?
acetyl-CoA + dibekacin	Corynebacterium striatum	-	CoA + N3-acetyldibekacin	-	?
acetyl-CoA + dibekacin	Corynebacterium striatum BM4687	-	CoA + N3-acetyldibekacin	-	?
acetyl-CoA + kanamycin B	Corynebacterium striatum	-	CoA + N3-acetylkanamycin B	-	?
acetyl-CoA + kanamycin B	Corynebacterium striatum BM4687	-	CoA + N3-acetylkanamycin B	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium striatum	-	strain isolated from the heel biopsy specimen of an 80-year-old diabetic woman	-
Corynebacterium striatum	A0A0K2X0F2	gene aac(3)-XI	-
Corynebacterium striatum BM4687	-	strain isolated from the heel biopsy specimen of an 80-year-old diabetic woman	-
Corynebacterium striatum BM4687	A0A0K2X0F2	gene aac(3)-XI	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3) by nickel affinity chromatography and ultrafiltration	Corynebacterium striatum

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-CoA + a 2-deoxystreptamine antibiotic	-	Corynebacterium striatum	CoA + N3-acetyl-2-deoxystreptamine antibiotic	-	?
acetyl-CoA + a 2-deoxystreptamine antibiotic	-	Corynebacterium striatum BM4687	CoA + N3-acetyl-2-deoxystreptamine antibiotic	-	?
acetyl-CoA + dibekacin	-	Corynebacterium striatum	CoA + N3''-acetyldibekacin	-	?
acetyl-CoA + dibekacin	-	Corynebacterium striatum BM4687	CoA + N3''-acetyldibekacin	-	?
acetyl-CoA + dibekacin	-	Corynebacterium striatum	CoA + N3-acetyldibekacin	-	?
acetyl-CoA + dibekacin	-	Corynebacterium striatum	CoA + N3-acetyldibekacin	product identification by high-field NMR	?
acetyl-CoA + dibekacin	-	Corynebacterium striatum BM4687	CoA + N3-acetyldibekacin	-	?
acetyl-CoA + dibekacin	-	Corynebacterium striatum BM4687	CoA + N3-acetyldibekacin	product identification by high-field NMR	?
acetyl-CoA + fortimicin	-	Corynebacterium striatum	CoA + N3-acetylfortimicin	-	?
acetyl-CoA + gentamicin C1A	-	Corynebacterium striatum	CoA + N3-acetylgentamicin C1A	-	?
acetyl-CoA + kanamycin B	-	Corynebacterium striatum	CoA + N3'-acetylkanamycin B	-	?
acetyl-CoA + kanamycin B	-	Corynebacterium striatum BM4687	CoA + N3'-acetylkanamycin B	-	?
acetyl-CoA + kanamycin B	-	Corynebacterium striatum	CoA + N3-acetylkanamycin B	-	?
acetyl-CoA + kanamycin B	-	Corynebacterium striatum BM4687	CoA + N3-acetylkanamycin B	-	?
acetyl-CoA + sisomicin	-	Corynebacterium striatum	CoA + N3-acetylsisomicin	-	?
acetyl-CoA + tobramycin	-	Corynebacterium striatum	CoA + N3-acetyltobramycin	-	?
additional information	the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin	Corynebacterium striatum	?	-	?
additional information	the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin	Corynebacterium striatum BM4687	?	-	?

Synonyms

Synonyms	Comment	Organism
AAC(3)-XI	-	Corynebacterium striatum
aminoglycoside 3-N-acetyltransferase type XI	-	Corynebacterium striatum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Corynebacterium striatum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.17	-	dibekacin	pH 6.0, 37°C	Corynebacterium striatum
0.17	-	dibekacin	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum
0.23	-	kanamycin B	pH 6.0, 37°C	Corynebacterium striatum
0.23	-	kanamycin B	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum
0.24	-	acetyl-CoA	pH 6.0, 37°C	Corynebacterium striatum
0.24	-	acetyl-CoA	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Corynebacterium striatum

Cofactor

Cofactor	Comment	Organism	Structure
acetyl-CoA	-	Corynebacterium striatum

General Information

General Information	Comment	Organism
evolution	aminoglycoside 3-N-acetyltransferases [AAC(3)] are widely distributed in Gram-negative bacteria and are categorized in five types (I, II, III, IV, and VI). To date, only four are identified in the Gram-positive aminoglycoside producers, overview	Corynebacterium striatum
physiological function	expression in Escherichia coli confers resistance to gentamicin, tobramycin, sisomicin, dibekacin, and fortimicin, the MICs of kanamycin A, netilmicin, amikacin, apramycin, and neomycin B remaining unchanged	Corynebacterium striatum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
17	-	kanamycin B	pH 6.0, 37°C	Corynebacterium striatum
17	-	kanamycin B	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum
18	-	dibekacin	pH 6.0, 37°C	Corynebacterium striatum
18	-	dibekacin	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum
89	-	acetyl-CoA	pH 6.0, 37°C	Corynebacterium striatum
89	-	acetyl-CoA	recombinant His-tagged enzyme, pH 6.0, 37°C	Corynebacterium striatum