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Literature summary for 2.3.1.81 extracted from

  • Magalhaes, M.L.; Blanchard, J.S.
    The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli (2005), Biochemistry, 44, 16275-16283.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the pET-23a(+) expression vector Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
desulfo-CoA
-
 Escherichia coli
netilmicin
-
 Escherichia coli
ribostamycin
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00015
-
 paromomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.00033
-
 sisomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.00036
-
 neomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.00067
-
 apramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.00097
-
 gentamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.00142
-
 tobramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.0031
-
 ribostamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.042
-
 netilmicin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.086
-
 acetyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.237
-
 butirosin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.238
-
 kanamycin A in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.477
-
 malonyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.499
-
 propionyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
1.16
-
 butyryl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
11.07
-
 amikacin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, enzyme in solution Escherichia coli
54000
-
 dynamic light scattering Escherichia coli
55750
-
 calculated from amino acid sequence Escherichia coli
60000
-
 Sephacryl-S200 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q306W4
-
-
Escherichia coli BL21(DE3) pLysS Q306W4
-
-

Purification (Commentary)

Purification (Comment) Organism
Fast Flow Q-Sepharose anion exchange column chromatography and Phenyl Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + amikacin
-
 Escherichia coli CoA + N3'-acetylamikacin
-
 ?
acetyl-CoA + amikacin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylamikacin
-
 ?
acetyl-CoA + apramycin
-
 Escherichia coli CoA + N3'-acetylapramycin
-
 ?
acetyl-CoA + apramycin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylapramycin
-
 ?
acetyl-CoA + butirosin
-
 Escherichia coli CoA + N3'-acetylbutirosin
-
 ?
acetyl-CoA + gentamicin
-
 Escherichia coli CoA + N3'-acetylgentamicin
-
 ?
acetyl-CoA + gentamicin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylgentamicin
-
 ?
acetyl-CoA + kanamycin
-
 Escherichia coli CoA + N3'-acetylkanamycin
-
 ?
acetyl-CoA + kanamycin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylkanamycin
-
 ?
acetyl-CoA + neomycin
-
 Escherichia coli CoA + ?
-
 ?
acetyl-CoA + netilmicin
-
 Escherichia coli CoA + N3'-acetylnetilmicin
-
 ?
acetyl-CoA + paromomycin
-
 Escherichia coli CoA + N3'-acetylparomomycin
-
 ?
acetyl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?
acetyl-CoA + sisomycin
-
 Escherichia coli CoA + N3'-acetylsisomycin
-
 ?
acetyl-CoA + tobramycin
-
 Escherichia coli CoA + N3'-acetyltobramycin
-
 ?
acetyl-CoA + tobramycin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetyltobramycin
-
 ?
butyryl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?
desulfo-CoA + ribostamycin
-
 Escherichia coli ?
-
 ?
malonyl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?
propionyl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, enzyme in solution Escherichia coli

Synonyms

Synonyms Comment Organism
AAC(3)-IV
-
 Escherichia coli
AAC3-IV aminoglycoside acetyltransferase
-
 Escherichia coli
aminoglycoside 3-N-acetyltransferase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 butyryl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
0.5
-
 amikacin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
3.5
-
 butirosin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
9
-
 malonyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
15
-
 paromomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
32
-
 netilmicin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
33
-
 propionyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
39
-
 acetyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
40
-
 gentamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
51
-
 sisomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
60
-
 neomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
61
-
 apramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
80
-
 kanamycin A in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
146
-
 ribostamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
162
-
 tobramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 corresponds to the plateau region of the pH profile, where the velocity is maximal and the variations in velocity due to changes in pH are smallest Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli calculated from amino acid sequence
-
 5.7