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Literature summary for 2.3.1.81 extracted from
- Purification and properties of an aminoglycoside acetyltransferase from Pseudomonas aeruginosa (1982), Biochemistry, 21, 871-875.
General Stability
| General Stability | Organism |
|---|---|
| enzyme activity is significantly stabilized when preparations contain substrate | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| kanamycin A | substrate inhibition | Pseudomonas aeruginosa |  |
| tobramycin | substrate inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000724 | - |
sisomicin | pH 7.2, 34°C | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
- |
Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax 0.102 (sisomicin), pH 7.2, 34°C | Pseudomonas aeruginosa |
| 9.743 | - |
pH 7.2, 34°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + sisomicin | - |
Pseudomonas aeruginosa | CoA + N3'-acetylsisomicin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAC(3) | - |
Pseudomonas aeruginosa |
| aminoglycoside 3-acetyltransferase | - |
Pseudomonas aeruginosa |
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