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Literature summary for 2.3.1.81 extracted from

  • Angelatou, F.; Litsas, S.B.; Kontomichalou, P.
    Purification and properties of two gentamicin-modifying enzymes, coded by a single plasmid pPK237 originating from Pseudomonas aeruginosa (1982), J. Antibiot., 35, 235-244.
    View publication on PubMed
Search for more literature for 2.3.1.81

Cloned(Commentary)

Cloned (Comment) Organism
Pseudomonas aeruginosa plasmid pPK237 transferred to and expressed in Escherichia coli K12 Pseudomonas aeruginosa

General Stability

General Stability Organism
dialysis against EDTA with subsequent Mg2+-addition to dialyzed enzyme has no effect on activity Pseudomonas aeruginosa
gel filtration leads to complete inactivation Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
EDTA above 0.5 mM Pseudomonas aeruginosa
additional information no inhibition by NH4+, K+, Na+, DTT, N-methylmaleimide Pseudomonas aeruginosa
Zn2+
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 gentamicin C1
-
 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.0 mM, about 100% activity enhancement Pseudomonas aeruginosa
Mg2+ 1.5 mM, about 100% activity enhancement Pseudomonas aeruginosa
NH4+ slight activation Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid analysis Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 plasmid pPK237 transferred to and expressed in Escherichia coli K12
-

Purification (Commentary)

Purification (Comment) Organism
plasmid pPK237 transferred to and expressed in E. coli K12, affinity chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
-
 Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-20°C, partially purified, several months Pseudomonas aeruginosa
-20°C, purified, several days Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + dibekacin acetylation at 8% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3''-acetyldibekacin
-
 ?
acetyl-CoA + gentamicin A acetylation at 6% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetylgentamicin A
-
 ?
acetyl-CoA + gentamicin C acetylation at 86% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetylgentamicin C
-
 ?
acetyl-CoA + gentamicin C1 best substrate Pseudomonas aeruginosa CoA + N3'-acetylgentamicin C1
-
 ?
acetyl-CoA + gentamicin C1a acetylation at 60% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetylgentamicin C1a
-
 ?
acetyl-CoA + gentamicin C2 acetylation at 84% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetylgentamicin C2
-
 ?
acetyl-CoA + kanamycin A acetylation at 7% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetylkanamycin A
-
 ?
acetyl-CoA + sisomicin acetylation at 51% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetylsisomicin
-
 ?
acetyl-CoA + tobramycin acetylation at 6% the rate of gentamicin C1 Pseudomonas aeruginosa CoA + N3'-acetyltobramycin
-
 ?
additional information no substrates are amikacin Pseudomonas aeruginosa ?
-
 ?
additional information lividomycin A, butirosin A and B Pseudomonas aeruginosa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 above, inactivation within 2 min Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.6
-
 Pseudomonas aeruginosa