Literature summary for 2.3.1.81 extracted from

Wolf, E.; Vassilev, A.; Makino, Y.; Sali, A.; Nakatani, Y.; Burley, S.K.
Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase (1998), Cell, 94, 439-449.

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Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and truncated enzymes expressed in Escherichia coli	Serratia marcescens

Crystallization (Commentary)

Crystallization (Comment)	Organism
X-ray structure of a recombinant enzyme, expressed in its selenomethionine substituted form, cocrystallization with 5 mM CoA at 4°C and pH: 7.8, crystal cryoprotection achieved with 17% glycerol and 8% 2R,3R-butanediol, active center found by X-ray analysis and model building	Serratia marcescens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Serratia marcescens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18630	-	18649 Da predicted from nucleotide sequence, 18630 Da found by mass spectrometry	Serratia marcescens

Organism

Organism	UniProt	Comment	Textmining
Serratia marcescens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
side-chain modification	cleavage of the hexahistidine tag by tobacco etch virus protease	Serratia marcescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + gentamicin	-	Serratia marcescens	CoA + N3'-acetylgentamicin	-	?

Synonyms

Synonyms	Comment	Organism
aminoglycoside 3-N-acetyltransferase	-	Serratia marcescens

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Release 2023.1 (January 2023)