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Literature summary for 2.3.1.8 extracted from

  • Dimou, M.; Venieraki, A.; Liakopoulos, G.; Katinakis, P.
    Cloning, characterization and transcriptional analysis of two phosphate acetyltransferase isoforms from Azotobacter vinelandii (2011), Mol. Biol. Rep., 38, 3653-3663.
    View publication on PubMed
Search for more literature for 2.3.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
acetyl phosphate substrate inhibition; substrate inhibition Azotobacter vinelandii
CoA substrate inhibition; substrate inhibition Azotobacter vinelandii
phosphate substrate inhibition; substrate inhibition Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 acetyl-CoA pH 7.2, 35°C Azotobacter vinelandii
0.2
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
0.3
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
0.5
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
1
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
11
-
 phosphate pH 7.2, 35°C Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, SDS-PAGE, recombinant protein Azotobacter vinelandii
77000
-
 x * 77000, SDS-PAGE, recombinant protein Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii C1DER7 isoform Pta-2
-
Azotobacter vinelandii C1DQG8 isoform Pta-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + CoA
-
 Azotobacter vinelandii acetyl-CoA + phosphate
-
 ?
acetyl phosphate + CoA with isoform Pta-2, reaction is irreversible, no acetyl phosphate forming reaction can be detected Azotobacter vinelandii acetyl-CoA + phosphate
-
 r
acetyl-CoA + phosphate
-
 Azotobacter vinelandii acetyl Phosphate + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE, recombinant protein Azotobacter vinelandii
? x * 77000, SDS-PAGE, recombinant protein Azotobacter vinelandii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
0.1
-
 CoA pH 7.2, 35°C Azotobacter vinelandii
5.2
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
12
-
 acetyl phosphate pH 7.2, 35°C Azotobacter vinelandii
19
-
 phosphate pH 7.2, 35°C Azotobacter vinelandii

Expression

Organism Comment Expression
Azotobacter vinelandii gene is expressed during exponential growth on glucose or acetate and is downregulated in the stationary phase additional information
Azotobacter vinelandii availabity of ammonium during growth on acetate results in upregulation up