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Literature summary for 2.3.1.8 extracted from
- Cloning of the phosphotransacetylase gene from Lactobacillus sanfranciscensis and characterization of its gene product (2001), J. Basic Microbiol., 41, 339-349.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Fructilactobacillus sanfranciscensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
CoA | - |
Fructilactobacillus sanfranciscensis |  |
| 0.6 | - |
acetyl-CoA | - |
Fructilactobacillus sanfranciscensis | |
| 1.3 | - |
acetyl phosphate | - |
Fructilactobacillus sanfranciscensis | |
| 6.7 | - |
phosphate | - |
Fructilactobacillus sanfranciscensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35500 | - |
calculated from amino acid sequence | Fructilactobacillus sanfranciscensis |
| 40000 | - |
SDS-PAGE | Fructilactobacillus sanfranciscensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | Fructilactobacillus sanfranciscensis | - |
CoA + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fructilactobacillus sanfranciscensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity, biotinylated fusion protein | Fructilactobacillus sanfranciscensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1940 | - |
- |
Fructilactobacillus sanfranciscensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Fructilactobacillus sanfranciscensis | CoA + acetyl phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | 58 | - |
Fructilactobacillus sanfranciscensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
inactivation after 15 min | Fructilactobacillus sanfranciscensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 9.1 | - |
Fructilactobacillus sanfranciscensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | stable | Fructilactobacillus sanfranciscensis |
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