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Literature summary for 2.3.1.8 extracted from
- Activation of acetate by Methanosarcina thermophila. Purification and characterization of phosphotransacetylase (1989), J. Biol. Chem., 264, 18392-18396.
General Stability
| General Stability | Organism |
|---|---|
| sulfate and phosphate partially protect against heat inactivation | Methanosarcina thermophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | above 10 mM | Methanosarcina thermophila |  |
| arsenate | - |
Methanosarcina thermophila | |
| Na+ | - |
Methanosarcina thermophila | |
| phosphate | end product inhibitor | Methanosarcina thermophila | |
| potassium phosphate | above 10 mM | Methanosarcina thermophila | |
| SO42- | - |
Methanosarcina thermophila | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Methanosarcina thermophila | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | K+ or NH4+ at concentration above 10 mM required for maximum activity | Methanosarcina thermophila | |
| NH4+ | above 10 mM | Methanosarcina thermophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
1 * 43000, SDS-PAGE | Methanosarcina thermophila |
| 52000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | Methanosarcina thermophila | - |
CoA + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina thermophila | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| stable to air | Methanosarcina thermophila |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina thermophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2469 | - |
- |
Methanosarcina thermophila |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable for at least 3 h when stored on ice | Methanosarcina thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + phosphate | - |
Methanosarcina thermophila | CoA + acetyl phosphate | - |
r | |
| acetyl-CoA + phosphate | rate of acetyl-CoA synthesis is 10times greater than rate of acetyl phosphate synthesis | Methanosarcina thermophila | CoA + acetyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Methanosarcina thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Methanosarcina thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
sulfate and phosphate partially protect against heat inactivation | Methanosarcina thermophila |
| 70 | - |
5 min, stable up to 70°C in absence of additional salts | Methanosarcina thermophila |
| 80 | - |
5 min, complete inactivation in absence of additional salts | Methanosarcina thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Methanosarcina thermophila |
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