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Literature summary for 2.3.1.8 extracted from

  • Whiteley, H.R.; Pelroy, R.A.
    Purification and properties of phosphotransacetylase from Veillonella alcalescens (1972), J. Biol. Chem., 247, 1911-1917.
    View publication on PubMed

General Stability

General Stability Organism
divalent cations, e.g. FeSO4, Fe(NH4)2SO4, MgCl2, MnCl2, MgSO4 or ATP increase lability Veillonella parvula
increased stability is obtained by adding a reducing agent and a component of the reaction Veillonella parvula

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Veillonella parvula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000 80000 gel filtration Veillonella parvula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + phosphate Veillonella parvula
-
CoA + acetyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Veillonella parvula
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Veillonella parvula

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
396
-
-
Veillonella parvula

Storage Stability

Storage Stability Organism
4°C or -20°C, protein concentration 0.5 mg/ml, several weeks Veillonella parvula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phosphate
-
Veillonella parvula CoA + acetyl phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000-40000, SDS-PAGE Veillonella parvula

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life: 20 min Veillonella parvula