Crystallization (Comment) | Organism |
---|---|
- |
Clostridium kluyveri |
General Stability | Organism |
---|---|
dilute solutions are instable, in frozen state stable | Clostridium kluyveri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | - |
Clostridium kluyveri | |
CuSO4 | - |
Clostridium kluyveri | |
K+ | - |
Clostridium kluyveri | |
KCN | weak | Clostridium kluyveri | |
Mg2+ | - |
Clostridium kluyveri | |
MnCl2 | weak | Clostridium kluyveri | |
Na+ | - |
Clostridium kluyveri | |
p-chloromercuribenzoate | - |
Clostridium kluyveri | |
Tris(hydroxymethyl)aminomethane | weak | Clostridium kluyveri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
CoA | - |
Clostridium kluyveri | |
0.66 | - |
acetyl phosphate | - |
Clostridium kluyveri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NH4+ | required for maximum activity, optimum at 7 mM (NH4)2SO4 | Clostridium kluyveri | |
phosphate | 1 mM, weak activation | Clostridium kluyveri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | 41000 | ultracentrifugation | Clostridium kluyveri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | Clostridium kluyveri | - |
CoA + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium kluyveri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium kluyveri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9100 | - |
after crystallization | Clostridium kluyveri |
Storage Stability | Organism |
---|---|
0-4°C, 2.7-3.0 M ammonium sulfate, months | Clostridium kluyveri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + phosphate | - |
Clostridium kluyveri | CoA + acetyl phosphate | - |
r | |
acetyl-CoA + phosphate | in reverse reaction specific for CoA | Clostridium kluyveri | CoA + acetyl phosphate | - |
r |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 40 | only small effect of temperature in this range | Clostridium kluyveri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, stable | Clostridium kluyveri |
45 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 15% loss of activity | Clostridium kluyveri |
50 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 48% loss of activity | Clostridium kluyveri |
60 | - |
5 min, 1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, pH 6, 98% loss of activity | Clostridium kluyveri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Clostridium kluyveri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.2 | about 50% of activity maximum at pH 6.6 pH and 8.2 | Clostridium kluyveri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
1.5 mg/ml protein concentration, 0.15 M ammonium sulfate, 40°C, 5 min, stable | Clostridium kluyveri |