Cloned (Comment) | Organism |
---|---|
cloning of the structural gene mac | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA-SH | product inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
acetyl-CoA | - |
Escherichia coli | |
40 | - |
triethanolamine | - |
Escherichia coli | |
62 | - |
D-glucose | - |
Escherichia coli | |
90 | - |
maltose | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, SDS-PAGE | Escherichia coli |
40000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + maltose | Escherichia coli | - |
CoA + 6-O-acetyl-alpha-D-glucopyranosyl-(1->4)-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.8 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, enzyme stored frozen in presence of 0.1% 2-mercaptoethanol and 40% glycerol without appreciable loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + D-fructose | - |
Escherichia coli | CoA + 6-O-acetyl-D-fructose | - |
? | |
acetyl-CoA + D-galactose | - |
Escherichia coli | CoA + 6-O-acetyl-D-galactose | - |
? | |
acetyl-CoA + D-glucose | - |
Escherichia coli | CoA + acetyl-D-glucose | - |
? | |
acetyl-CoA + D-mannose | - |
Escherichia coli | CoA + 6-O-acetyl-D-mannose | - |
? | |
acetyl-CoA + maltooligosaccharide | - |
Escherichia coli | CoA + ? | - |
? | |
acetyl-CoA + maltose | - |
Escherichia coli | CoA + 6-O-acetyl-alpha-D-glucopyranosyl-(1->4)-D-glucose | - |
? | |
acetyl-CoA + maltotriose | - |
Escherichia coli | CoA + 6-O-acetyl-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-D-glucopyranose | - |
? | |
acetyl-CoA + triethanolamine | - |
Escherichia coli | CoA + acetyl-triethanolamine | - |
? | |
additional information | rhamnose, arabinose, lactose, isopropyl-1-thio-beta-D-galactoside and trehalose are not substrates | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 20000, SDS-PAGE | Escherichia coli |