Cloned (Comment) | Organism |
---|---|
expression in HEK-293T cells | Bos taurus |
expression in Spodoptera frugiperda Sf9 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
all-trans-retinylbromoacetate | - |
Bos taurus | |
progesterone | - |
Bos taurus | |
XP620 | selective isozyme DGAT1 inhibitor, inhibits the diacylglycerol acyltransferase and the retinol acyltransferase activities at the retinol recognition site, inhibition of 85% of retinyl ester production in intact differentiated Caco-2 cells, IC50: 88.7 nM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bos taurus | |
additional information | - |
additional information | kinetics, recombinant enzyme | Homo sapiens | |
0.0041 | - |
all-trans-retinol | pH 7.5, 37°C, retinal pigment epithelium microsomes | Bos taurus | |
0.0041 | 0.0048 | all-trans-retinol | depending on assay method | Bos taurus | |
0.0087 | - |
11-cis-retinol | - |
Bos taurus | |
0.0087 | - |
11-cis-retinol | pH 7.5, 37°C, retinal pigment epithelium microsomes | Bos taurus | |
0.0139 | - |
palmitoyl-CoA | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
0.0259 | - |
retinol | pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Bos taurus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | ARAT may complement LRAT, EC 2.3.1.135, to provide additional retinyl-ester synthase activity under conditions of high all-trans-retinol | ? | - |
? | |
palmitoyl-CoA + 11-cis-retinol | Bos taurus | - |
CoA + 11-cis-retinyl palmitate | - |
r | |
palmitoyl-CoA + all-trans-retinol | Bos taurus | - |
CoA + all-trans-retinyl palmitate | - |
r | |
palmitoyl-CoA + retinol | Homo sapiens | - |
CoA + retinyl palmitate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + retinol = CoA + retinyl ester | enzyme mechanism and role in the visual cycle | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Caco-2 cell | - |
Homo sapiens | - |
eye | - |
Bos taurus | - |
liver | - |
Bos taurus | - |
retinal pigment epithelium | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ARAT may complement LRAT, EC 2.3.1.135, to provide additional retinyl-ester synthase activity under conditions of high all-trans-retinol | Bos taurus | ? | - |
? | |
additional information | the reaction is also catalyzed by the acyl coenzyme A:diacylglycerol acyltransferase, DGAT, EC 2.3.1.22 | Homo sapiens | ? | - |
? | |
palmitoyl-CoA + 11-cis-retinol | - |
Bos taurus | CoA + 11-cis-retinylpalmitate | - |
? | |
palmitoyl-CoA + 11-cis-retinol | - |
Bos taurus | CoA + 11-cis-retinyl palmitate | - |
r | |
palmitoyl-CoA + all-trans-retinol | - |
Bos taurus | CoA + all-trans-retinylpalmitate | - |
? | |
palmitoyl-CoA + all-trans-retinol | - |
Bos taurus | CoA + all-trans-retinyl palmitate | - |
r | |
palmitoyl-CoA + retinol | - |
Homo sapiens | CoA + retinyl palmitate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl CoA:retinol acyltransferase | - |
Bos taurus |
acyl coenzyme A:retinol acyltransferase | - |
Homo sapiens |
ArAT | - |
Homo sapiens |
ArAT | - |
Bos taurus |
DGAT1/ARAT | - |
Homo sapiens |
More | cf. EC 2.3.1.135 | Bos taurus |
More | cf. EC 2.3.1.22 | Homo sapiens |
retinyl-ester synthase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
37 | - |
in vivo and in vitro assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.5 | 8 | assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics depend highly on retinol concentration, overview, recombinant enzyme | Homo sapiens | |
0.000045 | - |
XP620 | pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000887 | - |
selective isozyme DGAT1 inhibitor, inhibits the diacylglycerol acyltransferase and the retinol acyltransferase activities at the retinol recognition site, inhibition of 85% of retinyl ester production in intact differentiated Caco-2 cells, IC50: 88.7 nM | Homo sapiens | XP620 |