Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.76 extracted from

  • Ross, A.C.
    Retinol esterification by rat liver microsomes. Evidence for a fatty acyl coenzyme A: retinol acyltransferase (1982), J. Biol. Chem., 257, 2453-2459.
    View publication on PubMed
Search for more literature for 2.3.1.76

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 0.02 mM enhances activity Rattus norvegicus
dithiothreitol 5 mM enhances activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate activity inhibited at 0.5% Rattus norvegicus
additional information not inhibited by cholesterol Rattus norvegicus
p-chloromercuribenzoate activity abolished at 5 mM Rattus norvegicus
progesterone activity inhibited above 0.05 mM Rattus norvegicus
sodium deoxycholate activity inhibited at 0.5% Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome membrane fraction Rattus norvegicus
-
-
microsome membrane fraction Felis silvestris
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + retinol Rattus norvegicus
-
 CoA + retinyl ester
-
 ?
acyl-CoA + retinol Felis silvestris
-
 CoA + retinyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Felis silvestris
-
 cat
-
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation Rattus norvegicus
partial purification by differential centrifugation Felis silvestris

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Felis silvestris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity determined from microsomes Rattus norvegicus
additional information
-
 activity determined from microsomes Felis silvestris

Storage Stability

Storage Stability Organism
-70ºC, phosphate buffer, pH 7.4, DTT, 2 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyl ester
-
 ?
acyl-CoA + retinol
-
 Felis silvestris CoA + retinyl ester
-
 ?
lauroyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyllaurate
-
 ?
lauroyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyllaurate
-
 ?
octanoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyloctanoate
-
 ?
octanoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyloctanoate
-
 ?
oleoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyloleolate
-
 ?
oleoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyloleolate
-
 ?
palmitoyl-CoA + retinol
-
 Felis silvestris CoA + retinylpalmitate
-
 ?
palmitoyl-CoA + retinol better effect with long chain fatty acids Rattus norvegicus CoA + retinylpalmitate
-
 ?
palmitoyl-CoA + retinol better effect with long chain fatty acids Rattus norvegicus Sprague-Dawley CoA + retinylpalmitate
-
 ?
stearoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinylstearate
-
 ?
stearoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinylstearate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 maximum esterification Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 maximum esterification between pH 7-8 Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 phosphate buffer Rattus norvegicus
7.4
-
 phosphate buffer Felis silvestris