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Literature summary for 2.3.1.75 extracted from

  • Xu, Y.; Pan, X.; Lu, J.; Wang, J.; Shan, Q.; Stout, J.; Chen, G.
    Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and diacylglycerol acyltransferase activity (2021), J. Exp. Bot., 72, 5584-5598.
    View publication on PubMed
Search for more literature for 2.3.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in in Saccharomyces cerevisiae strain H1246 and Chlamydomonas reinhardtii strain CC-5325 Chromochloris zofingiensis

Organism

Organism UniProt Comment Textmining
Chromochloris zofingiensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme displays a large preference towards palmitate over stearate by 4.5 and 4.3fold, respectively, when palmitoyl-CoA and oleoyl-CoA are used as acyl donors. Moreover, the enzyme shows a higher substrate specificity for oleoyl-CoA than palmitoyl-CoA, with enzyme displaying 2.2 and 2.3fold higher activity with oleoyl-CoA than palmitoyl-CoA when using palmitate and stearate as acyl acceptors, respectively Chromochloris zofingiensis ?
-
-
oleoyl-CoA + cetyl alcohol
-
 Chromochloris zofingiensis cetyl oleate + CoA
-
 ?
oleoyl-CoA + n-1,2-diolein weak activity, cf. EC 2.3.1.20 Chromochloris zofingiensis CoA + oleoyl-sn-1,2-diolein
-
 ?
oleoyl-CoA + palmitate
-
 Chromochloris zofingiensis palmitoyl oleate + CoA
-
 ?
oleoyl-CoA + palmitate
-
 Chromochloris zofingiensis stearoyl oleate + CoA
-
 ?
palmitoyl-CoA + palmitate
-
 Chromochloris zofingiensis palmitoyl palmitate + CoA
-
 ?
stearoyl-CoA + palmitate
-
 Chromochloris zofingiensis stearoyl palmitate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
MBOAT bifunctional enzyme with wax synthase and diacylglycerol acyltransferase activities Chromochloris zofingiensis
WS1
-
 Chromochloris zofingiensis

Expression

Organism Comment Expression
Chromochloris zofingiensis the enzyme is largely up-regulated during high light up

General Information

General Information Comment Organism
physiological function the enzyme displays both wax synthase and diacylglycerol acyltransferase activities and affects storage lipid accumulation when expressed in yeast and Chlamydomonas reinhardtii Chromochloris zofingiensis