Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.75 extracted from

  • Mancipe, N.C.; Mulliner, K.M.; Plunkett, M.H.; Barney, B.M.
    Canvasing the substrate-binding pockets of the wax ester synthase (2022), Biochemistry, 61, 922-932.
    View publication on PubMed
Search for more literature for 2.3.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TB1 cells Marinobacter nauticus

Protein Variants

Protein Variants Comment Organism
A144F the mutant shows increased activity with C8- to C14-CoA compared to the wild type enzyme Marinobacter nauticus
A144I the mutant shows increased activity with decanoyl-CoA compared to the wild type enzyme Marinobacter nauticus
A148I the mutant shows increased activity with octanoyl- and decanyol-CoA compared to the wild type enzyme Marinobacter nauticus
G25V the mutant shows reduced activity compared to the wild type enzyme Marinobacter nauticus
Q27F the mutant shows increased activity with decanoyl-CoA compared to the wild type enzyme Marinobacter nauticus
T132F the mutant shows increased activity with decanoyl-CoA compared to the wild type enzyme Marinobacter nauticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + a long-chain alcohol Marinobacter nauticus
-
 CoA + a long-chain ester
-
 ?

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus A1TX06 synonyms are Marinobacter aquaeolei VT8 and Marinobacter hydrocarbonoclasticus VT8
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating Sepharose column chromatography and amylose resin column chromatography Marinobacter nauticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + a long-chain alcohol
-
 Marinobacter nauticus CoA + a long-chain ester
-
 ?
decanoyl-CoA + dodecanol
-
 Marinobacter nauticus dodecyl decanoate + CoA
-
 ?
lauroyl-CoA + dodecanol
-
 Marinobacter nauticus dodecyl laurate + CoA
-
 ?
myristoyl-CoA + dodecanol
-
 Marinobacter nauticus dodecyl myristate + CoA
-
 ?
octanoyl-CoA + dodecanol
-
 Marinobacter nauticus dodecyl octanoate + CoA
-
 ?
palmitoyl-CoA + 1,3-linoleoyl-rac-glycerol
-
 Marinobacter nauticus ?
-
 ?
palmitoyl-CoA + 2-phenyl ethanol
-
 Marinobacter nauticus 2-phenylethyl palmitate + CoA
-
 ?
palmitoyl-CoA + decanol
-
 Marinobacter nauticus decyl palmitate + CoA
-
 ?
palmitoyl-CoA + dodecanol
-
 Marinobacter nauticus dodecyl palmitate + CoA
-
 ?
palmitoyl-CoA + ethanol
-
 Marinobacter nauticus ethyl palmitate + CoA
-
 ?
palmitoyl-CoA + heptadecanol
-
 Marinobacter nauticus heptadecyl palmitate + CoA
-
 ?
palmitoyl-CoA + hexadecanol
-
 Marinobacter nauticus hexadecyl palmitate + CoA
-
 ?
palmitoyl-CoA + hexanol
-
 Marinobacter nauticus hexyl palmitate + CoA
-
 ?
palmitoyl-CoA + isoamyl alcohol
-
 Marinobacter nauticus isoamyl palmitate + CoA
-
 ?
palmitoyl-CoA + n-hexanol
-
 Marinobacter nauticus hexyl palmitate + CoA
-
 ?
palmitoyl-CoA + nonanol
-
 Marinobacter nauticus nonyl palmitate + CoA
-
 ?
palmitoyl-CoA + octadecanol
-
 Marinobacter nauticus octadecyl palmitate + CoA
-
 ?
palmitoyl-CoA + octanol
-
 Marinobacter nauticus octyl palmitate + CoA
-
 ?
palmitoyl-CoA + tetradecanol
-
 Marinobacter nauticus tetradecyl palmitate + CoA
-
 ?
palmitoyl-CoA + tridecanol
-
 Marinobacter nauticus tridecyl palmitate + CoA
-
 ?
palmitoyl-CoA + undecanol
-
 Marinobacter nauticus undecyl palmitate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
acyl-coenzyme A:diacylglycerol acyltransferase
-
 Marinobacter nauticus
wax ester synthase
-
 Marinobacter nauticus
WS/DGAT
-
 Marinobacter nauticus