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Literature summary for 2.3.1.75 extracted from

  • Barney, B.M.; Wahlen, B.D.; Garner, E.; Wei, J.; Seefeldt, L.C.
    Differences in substrate specificities of five bacterial wax ester synthases (2012), Appl. Environ. Microbiol., 78, 5734-5745.
    View publication on PubMed
Search for more literature for 2.3.1.75

Application

Application Comment Organism
analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Marinobacter nauticus
analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Acinetobacter baylyi
analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Rhodococcus jostii
analysis development of an enzymic assay by indirect measurement of fatty alcohol and fatty acyl-CoA esterification catalyzed by the WS/DGAT enzyme using the coupled reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with free CoA liberated during the esterification reaction Psychrobacter cryohalolentis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter baylyi
expression in Escherichia coli Marinobacter nauticus
expression in Escherichia coli Psychrobacter cryohalolentis
expression in Escherichia coli Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi
-
-
-
Marinobacter nauticus
-
-
-
Psychrobacter cryohalolentis
-
-
-
Psychrobacter cryohalolentis K5
-
-
-
Rhodococcus jostii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
 substrate hexadecanol, pH 7.0, 22°C Acinetobacter baylyi
0.08
-
 substrate hexadecanol, pH 7.0, 22°C Psychrobacter cryohalolentis
0.12
-
 substrate hexadecanol, pH 7.0, 22°C Rhodococcus jostii
0.24
-
 substrate hexadecanol, isoform Ma2, pH 7.0, 22°C Marinobacter nauticus
0.68
-
 substrate hexadecanol, isoform Ma1, pH 7.0, 22°C Marinobacter nauticus
0.83
-
 substrate dodecanol, pH 7.0, 22°C Psychrobacter cryohalolentis
0.84
-
 substrate dodecanol, pH 7.0, 22°C Acinetobacter baylyi
3.37
-
 substrate dodecanol, pH 7.0, 22°C Rhodococcus jostii
4.96
-
 substrate dodecanol, isoform Ma2, pH 7.0, 22°C Marinobacter nauticus
37.3
-
 substrate dodecanol, isoform Ma1, pH 7.0, 22°C Marinobacter nauticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanol + palmitoyl-CoA and undecanol, best substrates Rhodococcus jostii decanyl palmitate + CoA
-
 ?
dodecanol + palmitoyl-CoA and undecanol, best substrates for both isoforms Ma1 and Ma2 Marinobacter nauticus dodecanyl palmitate + CoA
-
 ?
dodecanol + palmitoyl-CoA best substrate Acinetobacter baylyi dodecanyl palmitate + CoA
-
 ?
dodecanol + palmitoyl-CoA
-
 Rhodococcus jostii dodecanyl palmitate + CoA
-
 ?
dodecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis dodecanyl palmitate + CoA
-
 ?
dodecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 dodecanyl palmitate + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Marinobacter nauticus hexadecanyl palmitate + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Acinetobacter baylyi hexadecanyl palmitate + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis hexadecanyl palmitate + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 hexadecanyl palmitate + CoA
-
 ?
additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Marinobacter nauticus ?
-
 ?
additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Acinetobacter baylyi ?
-
 ?
additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Psychrobacter cryohalolentis ?
-
 ?
additional information the order of addition, i.e.the substrate that the protein is incubated with during the first minute while the background absorbance is measured, has a significant impact on the rate of reaction. No substrate: hexanol Psychrobacter cryohalolentis K5 ?
-
 ?
tetradecanol + palmitoyl-CoA
-
 Acinetobacter baylyi tetradecanyl palmitate + CoA
-
 ?
tetradecanol + palmitoyl-CoA best substrate Psychrobacter cryohalolentis tetradecanyl palmitate + CoA
-
 ?
tetradecanol + palmitoyl-CoA best substrate Psychrobacter cryohalolentis K5 tetradecanyl palmitate + CoA
-
 ?
undecanol + palmitoyl-CoA and dodecanol, best substrates for both isoforms Ma1 and Ma2 Marinobacter nauticus undecanyl palmitate + CoA
-
 ?
undecanol + palmitoyl-CoA
-
 Acinetobacter baylyi undecanyl palmitate + CoA
-
 ?
undecanol + palmitoyl-CoA and decanol, best substrates Rhodococcus jostii undecanyl palmitate + CoA
-
 ?
undecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis undecanyl palmitate + CoASH
-
 ?
undecanol + palmitoyl-CoA
-
 Psychrobacter cryohalolentis K5 undecanyl palmitate + CoASH
-
 ?

Synonyms

Synonyms Comment Organism
Rha1
-
 Rhodococcus jostii