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Literature summary for 2.3.1.75 extracted from

  • Kalscheuer, R.; Stoeveken, T.; Malkus, U.; Reichelt, R.; Golyshin, P.N.; Sabirova, J.S.; Ferrer, M.; Timmis, K.N.; Steinbuechel, A.
    Analysis of storage lipid accumulation in Alcanivorax borkumensis: evidence for alternative triacylglycerol biosynthesis routes in bacteria (2007), J. Bacteriol., 189, 918-928.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes atfA1 and atfA2, expression in Escherichia coli strain BL21(DE3) Alcanivorax borkumensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Alcanivorax borkumensis the enzyme plays the key role in the biosynthesis of triacylglycerols and wax esters, overview ?
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?

Organism

Organism UniProt Comment Textmining
Alcanivorax borkumensis
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a marine hydrocarbonoclastic bacterium, strain SK2 and several mutant strains, overview, genes atfA1, ABO_2742 and atfA2, ABO_1804, encoding isozymes AtfA1 and AtfA2
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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fatty acid content and fatty acid composition of Alcanivorax borkumensis strains and fatty acid composition of purified lipids isolated from strain SK2, overview Alcanivorax borkumensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme plays the key role in the biosynthesis of triacylglycerols and wax esters, overview Alcanivorax borkumensis ?
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?
additional information acyl acceptor specificities of isozymes AtfA1 and AtfA2 from A. borkumensis SK2 for glycerol and acylglycerols, overview Alcanivorax borkumensis ?
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?

Synonyms

Synonyms Comment Organism
AtfA1
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Alcanivorax borkumensis
wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase
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Alcanivorax borkumensis
WS/DGAT
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Alcanivorax borkumensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
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assay at Alcanivorax borkumensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
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assay at Alcanivorax borkumensis