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Literature summary for 2.3.1.75 extracted from

  • Holtzapple, E.; Schmidt-Dannert, C.
    Biosynthesis of isoprenoid wax ester in Marinobacter hydrocarbonoclasticus DSM 8798: identification and characterization of isoprenoid coenzyme A synthetase and wax ester synthases (2007), J. Bacteriol., 189, 3804-3812.
    View publication on PubMed
Search for more literature for 2.3.1.75

Cloned(Commentary)

Cloned (Comment) Organism
screening, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Marinobacter nauticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of isozymes WS1 and WS2 Marinobacter nauticus
0.044
-
 hexadecanol pH 7.4, 37°C, isozyme WS2 Marinobacter nauticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + long-chain fatty alcohol Marinobacter nauticus
-
 CoA + long-chain fatty ester
-
 ?
additional information Marinobacter nauticus the enzyme is involved in synthesis of isoprenoid wax ester storage compounds, pathway, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus
-
 DSM 8798, isozymes WS1, WS2, and WS3
-
Marinobacter nauticus
-
 DSM 8798
-

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + a long-chain alcohol = CoA + a long-chain ester the catalytic domain contains the highly conserved HHXXXDG motif Marinobacter nauticus
a

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cells grown on phytol as the sole carbon source under limiting nitrogen and/or phosphorous conditions Marinobacter nauticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + long-chain fatty alcohol
-
 Marinobacter nauticus CoA + long-chain fatty ester
-
 ?
additional information the enzyme is involved in synthesis of isoprenoid wax ester storage compounds, pathway, overview Marinobacter nauticus ?
-
 ?
additional information substrate profiles of isozymes WS1 and WS2, acyl-CoA specificity of isozyme WS2 with preference for palmitoyl-CoA and broad activity with alcohols of various chain lengths, bifunctional enzyme also catalyzing the reaction of EC 2.3.1.20 Marinobacter nauticus ?
-
 ?
oleoyl-CoA + 1,2-dipalmitoyl-sn-glycerol
-
 Marinobacter nauticus CoA + 1,2-dipalmitoyl-3-oleoyl-sn-glycerol
-
 ?
palmitoyl-CoA + hexadecanol
-
 Marinobacter nauticus CoA + hexadecyl palmitate
-
 ?
palmitoyl-CoA + phytol
-
 Marinobacter nauticus phytyl palmitate + CoA
-
 ?
phytanoyl-CoA + phytol
-
 Marinobacter nauticus ?
-
 ?

Synonyms

Synonyms Comment Organism
isoprenoid wax ester synthase
-
 Marinobacter nauticus
additional information cf. EC 2.3.1.20 Marinobacter nauticus
wax ester synthase
-
 Marinobacter nauticus
wax ester synthase/diacylglycerol acyltransferase
-
 Marinobacter nauticus
WS
-
 Marinobacter nauticus
WS/DGAT
-
 Marinobacter nauticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Marinobacter nauticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4794
-
 hexadecanol pH 7.4, 37°C, isozyme WS2 Marinobacter nauticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Marinobacter nauticus