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Literature summary for 2.3.1.75 extracted from

  • Stoveken, T.; Kalscheuer, R.; Malkus, U.; Reichelt, R.; Steinbuchel, A.
    The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase (2005), J. Bacteriol., 187, 1369-1376.
    View publication on PubMed
Search for more literature for 2.3.1.75

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate 45 mM, 10% increase in activtiy Acinetobacter baylyi ADP1

General Stability

General Stability Organism
decrease in activity with buffers such as Tris, Bis-Tris, HEPES, morpholinoethanesulfonic acid Acinetobacter baylyi ADP1
highly sensitive to proteases, especially to metallorpoteases, proteolysis is inhibited by addition of a cocktail of protease inhibitors including EDTA Acinetobacter baylyi ADP1

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1
MgCl2 5 mM, 17% inhibition Acinetobacter baylyi ADP1
MnCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 palmitoyl-CoA pH 7.4, 35°C Acinetobacter baylyi ADP1

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm partially Acinetobacter baylyi ADP1 5737
-
lipid droplet
-
 Acinetobacter baylyi ADP1 5811
-
membrane cytoplasm membrane Acinetobacter baylyi ADP1 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000, SDS-PAGE Acinetobacter baylyi ADP1
94100
-
 gel filtration Acinetobacter baylyi ADP1

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi ADP1 Q8GGG1 bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp., enzyme also has acyl-CoA:monoacylglycerol acyltransferase activity, expression in Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 pH 7.4, 35°C Acinetobacter baylyi ADP1

Storage Stability

Storage Stability Organism
-70°C, several months without significant loss of activity Acinetobacter baylyi ADP1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-decanol + palmitoyl-CoA 48% the rate of hexacedanol Acinetobacter baylyi ADP1 decyl palmitate + CoA
-
 ?
2-cyclohexylethanol + palmitoyl-CoA 44.8% the rate of hexacedanol Acinetobacter baylyi ADP1 2-cyclohexylethyl palmitate + CoA
-
 ?
2-decanol + palmitoyl-CoA 38.8% the rate of hexacedanol Acinetobacter baylyi ADP1 dec-2-yl palmitate + CoA
-
 ?
4-decanol + palmitoyl-CoA 15.6% the rate of hexacedanol Acinetobacter baylyi ADP1 4-decyl palmitate + CoA
-
 ?
cyclododecanol + palmitoyl-CoA 79.7% the rate of hexacedanol Acinetobacter baylyi ADP1 cyclododecyl palmitate + CoA
-
 ?
cyclohexandiol + palmitoyl-CoA 4.1% the rate of hexacedanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
cyclohexanol + palmitoyl-CoA 32% the rate of hexacedanol Acinetobacter baylyi ADP1 cyclohexyl palmitate + CoA
-
 ?
cyclohexanone oxime + palmitoyl-CoA 5.2% the rate of hexacedanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
hexadecanol + palmitoyl-CoA
-
 Acinetobacter baylyi ADP1 hexadecyl palmitate + CoA
-
 ?
additional information no substrate: resorcinol Acinetobacter baylyi ADP1 ?
-
 ?
phenol + palmitoyl-CoA 4.1% the rate of hexacedanol Acinetobacter baylyi ADP1 phenyl palmitate + CoA
-
 ?
phenylethanol + palmitoyl-CoA 99% the rate of hexacedanol Acinetobacter baylyi ADP1 phenylethyl palmitate + CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Acinetobacter baylyi ADP1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 45
-
 Acinetobacter baylyi ADP1
65
-
 no activity above Acinetobacter baylyi ADP1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 40 stable Acinetobacter baylyi ADP1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 phosphate buffer Acinetobacter baylyi ADP1