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Literature summary for 2.3.1.75 extracted from

  • Lardizabal, K.D.; Metz, J.G.; Sakamoto, T.; Hutton, W.C.; Pollard, M.R.; Lassner, M.W.
    Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic Arabidopsis (2000), Plant Physiol., 122, 645-655.
    View publication on PubMed
Search for more literature for 2.3.1.75

Application

Application Comment Organism
agriculture transgenic plants expressing the enzyme allow the production of long-chain liquid waxes at reasonable cost for use in commercial applications Simmondsia chinensis

Cloned(Commentary)

Cloned (Comment) Organism
cloning of cDNA, DNA sequence analysis, construction of plasmid to express the cDNA in transgenic Arabidopsis thaliana plants Simmondsia chinensis

Protein Variants

Protein Variants Comment Organism
additional information cloned cDNA is expressed in transgenic Arabidopsis thaliana plants, in combination with cDNAs encoding the jojoba fatty acyl-CoA reductase and a beta-ketoacyl-CoA synthase from Lunaria annua under a seed specific promotor, highly enhanced wax content in transgenic seeds Simmondsia chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Simmondsia chinensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence analysis and alignment Simmondsia chinensis
30000
-
 x * 30000, SDS-PAGE Simmondsia chinensis
40000
-
 gel filtration Simmondsia chinensis

Organism

Organism UniProt Comment Textmining
Simmondsia chinensis Q9XGY6
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Simmondsia chinensis

Source Tissue

Source Tissue Comment Organism Textmining
embryo developing Simmondsia chinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eicosenoyl-CoA + octadecenol best substrates Simmondsia chinensis octadecenyl eicosenoate + CoA
-
 ?
additional information substrate specificity, overview Simmondsia chinensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Simmondsia chinensis

Synonyms

Synonyms Comment Organism
fatty acyl-coenzyme A:fatty alcohol acyltransferase
-
 Simmondsia chinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 9.8 Simmondsia chinensis