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Literature summary for 2.3.1.74 extracted from
- Chalcone synthase from cell suspension cultures of phaseolus vulgaris L. (1983), Biochim. Biophys. Acta, 747, 298-303.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 2fold increase at 20 mM | Phaseolus vulgaris |  |
| dithiothreitol | 4fold increase at 20 mM | Phaseolus vulgaris | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-hydroxygenistein | - |
Phaseolus vulgaris | |
| cerulenin | - |
Phaseolus vulgaris | |
| Dalbergioidin | - |
Phaseolus vulgaris | |
| isoliquiritigenin | - |
Phaseolus vulgaris | |
| Kievitone | 50% inhibition at 0.001 mM | Phaseolus vulgaris | |
| naringenin | - |
Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
4-coumaroyl-CoA | - |
Phaseolus vulgaris | |
| 0.0214 | - |
malonyl-CoA | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 31fold | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00176 | - |
- |
Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + 4-coumaroyl-CoA | - |
Phaseolus vulgaris | naringenin chalcone + CoA + CO2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Phaseolus vulgaris |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0207 | - |
Kievitone | - |
Phaseolus vulgaris | |
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