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Literature summary for 2.3.1.74 extracted from

  • Ozeki, Y.; Sakano, K.; Komamine, A.; Tanaka, Y.; Noguchi, H.; Sankawa, U.; Suzuki, T.
    Purification and some properties of chalcone synthase from a carrot suspension culture induced for anthocyanin synthesis and preparation of its specific antiserum (1985), J. Biochem., 98, 9-17.
    View publication on PubMed
Search for more literature for 2.3.1.74

Inhibitors

Inhibitors Comment Organism Structure
Tris
-
 Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0057
-
 4-coumaroyl-CoA
-
 Daucus carota
0.018
-
 malonyl-CoA
-
 Daucus carota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 2 * 46000, SDS-PAGE Daucus carota
80000 85000 gel filtration, PAGE under non-denaturing conditions Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
 carrot, var Kurdagosun
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0188
-
-
 Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 4-coumaroyl-CoA
-
 Daucus carota naringenin chalcone + CoA + CO2 chalcone is the initial product that spontaneously transforms to naringenin ?

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 4-coumaroyl-CoA Daucus carota