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Literature summary for 2.3.1.74 extracted from

  • Lanz, T.; Tropf, S.; Marner, F.J.; Schröder, J.; Schröder, G.
    The role of cysteines in polyketide synthases. Site-directed mutagenesis of resveratrol and chalcone synthases, two key enzymes in different plant-specific pathways (1991), J. Biol. Chem., 266, 9971-9976.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, site directed mutagenesis, sequence alignment with EC 2.3.1.95 Sinapis alba

Protein Variants

Protein Variants Comment Organism
C135A 77% enzyme activity Sinapis alba
C169A no enzyme activity Sinapis alba
C195A increased enzyme activity Sinapis alba
C347A 44% enzyme activity Sinapis alba
C65A 15% enzyme activity Sinapis alba
C89A 71% enzyme activity Sinapis alba

Inhibitors

Inhibitors Comment Organism Structure
cerulenin 50% inhibition at 0.001 mg per assay Sinapis alba

Organism

Organism UniProt Comment Textmining
Sinapis alba
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 4-coumaroyl-CoA
-
 Sinapis alba naringenin chalcone + CoA + CO2 due to the presence of isomerase the immediate product naringenin chalcone is not detectable, instead 4,2',4',6'-tetrahydroxychalcone is found, the latter cyclizes spontaneously to naringenin ?