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Literature summary for 2.3.1.72 extracted from
- Partial purification and characterization of indol-3-ylacetylglucoe:myo-inositol indol-3-ylacetyltransferase (indoleacetic acid-inositol synthase) (1990), Plant Physiol., 94, 1598-1604.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization, stable to | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 74% inhibition at 5 mM | Zea mays |  |
| Dithionite | inhibition at 40 mM | Zea mays | |
| dithiothreitol | 98% inhibition at 5 mM | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
1-O-indol-3-yl-acetyl-beta-D-glucose | - |
Zea mays | |
| 4.1 | - |
myo-inositol | - |
Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-acetyl-beta-1-D-glucoside + myo-inositol | Zea mays | involved in biosynthesis of indole-3-acetyl-beta-1,4-glucan | D-glucose + indole-3-acetyl-myo-inositol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
sweet corn, var. Seneca Horizon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kernel | endosperm | Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.3 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude liquid endosperm, several years, no loss of activity | Zea mays |
| 4°C, more than 2 months | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-acetyl-beta-1-D-glucoside + myo-inositol | scyllo-inositol or myo-inosose-2 can replace myo-inositol | Zea mays | D-glucose + indole-3-acetyl-myo-inositol | - |
? | |
| indole-3-acetyl-beta-1-D-glucoside + myo-inositol | myo-inositol-D-galactopyranose, cyclohexanol, mannitol, glycerol | Zea mays | D-glucose + indole-3-acetyl-myo-inositol | - |
? | |
| indole-3-acetyl-beta-1-D-glucoside + myo-inositol | involved in biosynthesis of indole-3-acetyl-beta-1,4-glucan | Zea mays | D-glucose + indole-3-acetyl-myo-inositol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.1 | Zea mays |
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