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Literature summary for 2.3.1.71 extracted from

  • Webster, L.T.
    Benzoyl-CoA: amino acid and phenylacetyl-CoA: amino acid N-acyltransferases (1981), Methods Enzymol., 77, 301-308.
    View publication on PubMed
Search for more literature for 2.3.1.71

Inhibitors

Inhibitors Comment Organism Structure
Mg2+
-
 Bos taurus
Ni2+
-
 Bos taurus
Zn2+
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 1 * 33000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
benzoyl-CoA + glycine = CoA + hippurate sequential reaction mechanism in which the acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves and the peptide product dissociates last Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, 3 weeks, enzyme retains about 80% of its initial activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcrotonyl-CoA + glycine
-
 Bos taurus CoA + N-3-methylcrotonylglycine
-
 ?
acetyl-CoA + glycine
-
 Bos taurus CoA + N-acetylglycine
-
 ?
benzoyl-CoA + glycine
-
 Bos taurus CoA + N-benzoylglycine
-
 ?
benzoyl-CoA + L-asparagine weak activity Bos taurus CoA + N-benzoylasparagine
-
 ?
isobutyryl-CoA + glycine
-
 Bos taurus CoA + N-isobutyrylglycine
-
 ?
isovaleryl-CoA + glycine
-
 Bos taurus CoA + N-isovalerylglycine
-
 ?
n-butyryl-CoA + glycine
-
 Bos taurus CoA + N-butyrylglycine
-
 ?
propionyl-CoA + glycine
-
 Bos taurus CoA + N-propionylglycine
-
 ?
salicyl-CoA + glycine
-
 Bos taurus CoA + salicyluric acid
-
 ?
tiglyl-CoA + glycine
-
 Bos taurus CoA + N-tiglylglycine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.6
-
 Bos taurus