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Literature summary for 2.3.1.7 extracted from

  • Hsiao, Y.S.; Jogl, G.; Tong, L.
    Crystal structures of murine carnitine acetyltransferase in ternary complexes with its substrates (2006), J. Biol. Chem., 281, 28480-28487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.7

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, high resolution crystal structure of wild-type murine carnitine acetyltransferase in a ternary complex with its substrates acetyl-CoA and carnitine, and the structure of the S554A/M564G double mutant in a ternary complex with the substrates CoA and hexanoylcarnitine Mus musculus

Protein Variants

Protein Variants Comment Organism
H343A mutant enzymje shows no activity with acetyl-CoA and hexanoyl-CoA Mus musculus
H343E kcat/KM for acetyl-CoA is 9.8fold lower than wild-type value, mutant enzyme shows mo activity with hexanoyl-CoA as substrate Mus musculus
M564G kcat/KM for acetyl-CoA is 12.4fold lower than wild-type value, kcat/Km for hexanoyl-CoA is 31.4fold higher than wild-type value Mus musculus
S554A kcat/KM for acetyl-CoA is 109fold lower than wild-type value, kcat/Km for hexanoyl-CoA is 8fold lower than wild-type value Mus musculus
S554A/M564G kcat/KM for acetyl-CoA is 196fold lower than wild-type value, kcat/Km for hexanoyl-CoA is 4fold lower than wild-type value Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 acetyl-CoA 21°C, pH 7.8, wild-type enzyme Mus musculus
0.0224
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme M564G Mus musculus
0.0324
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme H343E Mus musculus
0.0415
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme S554A Mus musculus
0.0596
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme S554A/M564G Mus musculus
0.0746
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme M564G Mus musculus
0.0921
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme S554A Mus musculus
0.1034
-
 hexanoyl-CoA 21°C, pH 7.8, wild-type enzyme Mus musculus
0.112
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme S554A/M564G Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P47934
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
 Mus musculus CoA + O-acetylcarnitine
-
 ?
hexanoyl-CoA + carnitine
-
 Mus musculus hexanoylcarnitine + CoASH
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 acetyl-CoA 21°C, pH 7.8, wild-type enzyme Mus musculus
1.4
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme S554A/M564G Mus musculus
1.42
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme S554A Mus musculus
4
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme S554A Mus musculus
8.2
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme S554A/M564G Mus musculus
15.4
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme H343E Mus musculus
28.4
-
 acetyl-CoA 21°C, pH 7.8, mutant enzyme M564G Mus musculus
30.4
-
 hexanoyl-CoA 21°C, pH 7.8, wild-type enzyme Mus musculus
89
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme M564G Mus musculus
97.6
-
 acetyl-CoA 21°C, pH 7.8, wild-type enzyme Mus musculus
104.7
-
 hexanoyl-CoA 21°C, pH 7.8, mutant enzyme M564G Mus musculus