Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystallized in the presence of L-carnitine by vapor diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F545A | increases Km for both substrates | Homo sapiens |
F545Y | minor effects on Km for both substrates | Homo sapiens |
R497Q | increases Km for both substrates | Homo sapiens |
T444A | increases Km for both substrates | Homo sapiens |
Y431A | increases Km for both substrates | Homo sapiens |
Y431F | increases Km for both substrates | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
acetyl-CoA | wild type enzyme, pH 8.0 | Homo sapiens | |
0.064 | - |
acetyl-CoA | F545Y mutant enzyme, pH 8.0 | Homo sapiens | |
0.087 | - |
acetyl-CoA | Y431A mutant enzyme, pH 8.0 | Homo sapiens | |
0.11 | - |
L-carnitine | wild type enzyme, pH 8.0 | Homo sapiens | |
0.12 | - |
acetyl-CoA | R497Q mutant enzyme, pH 8.0 | Homo sapiens | |
0.15 | - |
L-carnitine | F545Y mutant enzyme, pH 8.0 | Homo sapiens | |
0.178 | - |
acetyl-CoA | F545A mutant enzyme, pH 8.0 | Homo sapiens | |
0.188 | - |
acetyl-CoA | T444A mutant enzyme, pH 8.0 | Homo sapiens | |
0.498 | - |
acetyl-CoA | Y431F mutant enzyme, pH 8.0 | Homo sapiens | |
2 | - |
L-carnitine | F545A mutant enzyme, pH 8.0 | Homo sapiens | |
7.5 | - |
L-carnitine | T444A mutant enzyme, pH 8.0 | Homo sapiens | |
11.1 | - |
L-carnitine | Y431A mutant enzyme, pH 8.0 | Homo sapiens | |
25.6 | - |
L-carnitine | R497Q mutant enzyme, pH 8.0 | Homo sapiens | |
35.9 | - |
L-carnitine | Y431F mutant enzyme, pH 8.0 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
mitochondrial matrix | - |
Homo sapiens | 5759 | - |
peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + carnitine | Homo sapiens | modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism | CoA + O-acetylcarnitine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P43155 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + carnitine | - |
Homo sapiens | CoA + O-acetylcarnitine | - |
r | |
acetyl-CoA + carnitine | modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism | Homo sapiens | CoA + O-acetylcarnitine | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | crystal structure analysis | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
acetyl-CoA | Y431F mutant enzyme, pH 8.0 | Homo sapiens | |
5.76 | - |
acetyl-CoA | T444A mutant enzyme, pH 8.0 | Homo sapiens | |
8.57 | - |
acetyl-CoA | F545A mutant enzyme, pH 8.0 | Homo sapiens | |
30.5 | - |
acetyl-CoA | F545Y mutant enzyme, pH 8.0 | Homo sapiens | |
38.5 | - |
acetyl-CoA | wild type enzyme, pH 8.0 | Homo sapiens | |
44.2 | - |
acetyl-CoA | Y431A mutant enzyme, pH 8.0 | Homo sapiens | |
94.8 | - |
acetyl-CoA | R497Q mutant enzyme, pH 8.0 | Homo sapiens |