Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae and Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E347A | complete loss of activity | Rattus norvegicus |
H343A | complete loss of activity | Rattus norvegicus |
M564A | lowered activity with acetyl-CoA, increased activity with longer chain acyl-CoAs | Rattus norvegicus |
M564G | lowered activity with acetyl-CoA, increased activity with longer chain acyl-CoAs, 1250-fold increase in activity with myristoyl-CoA | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
octanoyl-CoA | M564G mutant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.015 | - |
butyryl-CoA | M564G mutant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.022 | - |
hexanoyl-CoA | M564G mutant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.023 | - |
acetyl-CoA | recombinant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.025 | - |
octanoyl-CoA | recombinant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.03 | - |
butyryl-CoA | recombinant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.032 | - |
acetyl-CoA | M564G mutant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.033 | - |
hexanoyl-CoA | recombinant enzyme from yeast, pH 7.8, 30°C | Rattus norvegicus | |
0.086 | - |
carnitine | M564G mutant enzyme from yeast with octanoyl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.164 | - |
carnitine | M564G mutant enzyme from yeast with hexanoyl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.17 | - |
carnitine | M564G mutant enzyme from yeast with butyryl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.203 | - |
carnitine | recombinant enzyme from yeast with acetyl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.22 | - |
carnitine | recombinant enzyme from yeast with butyryl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.339 | - |
carnitine | M564G mutant enzyme from yeast with acetyl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.567 | - |
carnitine | recombinant enzyme from yeast with hexanoyl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus | |
0.964 | - |
carnitine | recombinant enzyme from yeast with octanoyl-CoA as second substrate, pH 7.8, 30°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70800 | - |
x * 70800, deduced from amino acid sequence | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + carnitine | Rattus norvegicus | modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism | CoA + O-acetylcarnitine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q704S8 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of GST-fusion proteins from Escherichia coli | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + carnitine | - |
Rattus norvegicus | CoA + O-acetylcarnitine | - |
r | |
acetyl-CoA + carnitine | modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism | Rattus norvegicus | CoA + O-acetylcarnitine | - |
r | |
butyryl-CoA + carnitine | - |
Rattus norvegicus | CoA + O-butyrylcarnitine | - |
r | |
hexanoyl-CoA + carnitine | very low activity | Rattus norvegicus | CoA + O-hexanoylcarnitine | - |
r | |
additional information | wild type enzyme is almost inactive towards longer chain fatty acids, some mutations increases activity towards longer chain fatty acids | Rattus norvegicus | ? | - |
? | |
octanoyl-CoA + carnitine | very low activity | Rattus norvegicus | CoA + O-octanoylcarnitine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 70800, deduced from amino acid sequence | Rattus norvegicus |