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Literature summary for 2.3.1.7 extracted from

  • Mittal, B.; Kurup, C.K.R.
    Purification of clofibrate-induced carnitine acetyltransferase from rat liver mitochondria (1980), Biochim. Biophys. Acta, 619, 90-97.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Clofibrate
-
Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Columba sp.

General Stability

General Stability Organism
retains activity indefinitely when kept frozen, pure enzyme retains activity when kept frozen for long periods of time, inactivation of pure preparations occurs during dialysis Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
acetylcarnitine competitive inhibition, inhibitor for the forward reaction Rattus norvegicus
acetylcarnitine
-
Sus scrofa
DL-carnitine inhibitor for the reverse reaction Rattus norvegicus
iodoacetamide
-
Rattus norvegicus
iodoacetamide
-
Sus scrofa
N-ethylmaleimide
-
Rattus norvegicus
N-ethylmaleimide
-
Sus scrofa
p-hydroxymercuribenzoate
-
Rattus norvegicus
p-hydroxymercuribenzoate
-
Sus scrofa
palmitoyl-CoA
-
Columba sp.
palmitoyl-CoA
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
CoASH
-
Rattus norvegicus
0.037
-
CoASH
-
Columba sp.
0.038
-
butyryl-CoA
-
Rattus norvegicus
0.12
-
L-(-)-carnitine
-
Columba sp.
0.28
-
acetylcarnitine
-
Rattus norvegicus
0.72
-
DL-carnitine
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Columba sp.
-
-
microsome membrane bound Rattus norvegicus
-
-
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
34000
-
1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
55000
-
gel filtration Columba sp.
56000
-
gel filtration Rattus norvegicus
75000
-
SDS-PAGE Columba sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + carnitine Rattus norvegicus
-
O-acetylcarnitine + CoASH
-
r
acetyl-CoA + carnitine Sus scrofa
-
O-acetylcarnitine + CoASH
-
r
acetyl-CoA + carnitine Columba sp.
-
O-acetylcarnitine + CoASH
-
r
acyl-CoA + carnitine Rattus norvegicus
-
acylcarnitine + CoASH
-
r
acyl-CoA + carnitine Sus scrofa
-
acylcarnitine + CoASH
-
r
acyl-CoA + carnitine Columba sp.
-
acylcarnitine + CoASH
-
r
acylcarnitine + CoASH Rattus norvegicus
-
acyl-CoA + carnitine
-
r
acylcarnitine + CoASH Sus scrofa
-
acyl-CoA + carnitine
-
r
acylcarnitine + CoASH Columba sp.
-
acyl-CoA + carnitine
-
r
O-acetylcarnitine + CoASH Rattus norvegicus
-
acetyl-CoA + carnitine
-
r
O-acetylcarnitine + CoASH Sus scrofa
-
acetyl-CoA + carnitine
-
r
O-acetylcarnitine + CoASH Columba sp.
-
acetyl-CoA + carnitine
-
r

Organism

Organism UniProt Comment Textmining
Columba sp.
-
-
-
Rattus norvegicus
-
rat
-
Rattus norvegicus
-
albino
-
Sus scrofa
-
swine
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Columba sp.
partially Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
breast muscle
-
Columba sp.
-
heart
-
Rattus norvegicus
-
heart
-
Sus scrofa
-
heart
-
Columba sp.
-
liver
-
Rattus norvegicus
-
liver
-
Columba sp.
-
muscle
-
Rattus norvegicus
-
muscle
-
Columba sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
82.83
-
-
Rattus norvegicus
85
-
-
Rattus norvegicus
85
-
-
Columba sp.

Storage Stability

Storage Stability Organism
0-4°C, loses little activity when stored for months Rattus norvegicus
0-4°C, loses little activity when stored for months Columba sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + carnitine
-
Rattus norvegicus O-acetylcarnitine + CoASH
-
r
acetyl-CoA + carnitine
-
Sus scrofa O-acetylcarnitine + CoASH
-
r
acetyl-CoA + carnitine
-
Columba sp. O-acetylcarnitine + CoASH
-
r
acetyl-CoA + L-(-)carnitine
-
Rattus norvegicus O-acetylcarnitine + CoASH
-
r
acetyl-CoA + L-(-)carnitine
-
Sus scrofa O-acetylcarnitine + CoASH
-
r
acetyl-CoA + L-(-)carnitine
-
Columba sp. O-acetylcarnitine + CoASH
-
r
acyl-CoA + carnitine
-
Rattus norvegicus acylcarnitine + CoASH
-
r
acyl-CoA + carnitine
-
Sus scrofa acylcarnitine + CoASH
-
r
acyl-CoA + carnitine
-
Columba sp. acylcarnitine + CoASH
-
r
acylcarnitine + CoASH
-
Rattus norvegicus acyl-CoA + carnitine
-
r
acylcarnitine + CoASH
-
Sus scrofa acyl-CoA + carnitine
-
r
acylcarnitine + CoASH
-
Columba sp. acyl-CoA + carnitine
-
r
additional information
-
Sus scrofa ?
-
?
additional information
-
Columba sp. ?
-
?
additional information palmityl-CoA does not act as a substrate for the liver enzyme Rattus norvegicus ?
-
?
O-acetylcarnitine + CoASH
-
Rattus norvegicus acetyl-CoA + L-(-)carnitine
-
r
O-acetylcarnitine + CoASH
-
Sus scrofa acetyl-CoA + L-(-)carnitine
-
r
O-acetylcarnitine + CoASH
-
Columba sp. acetyl-CoA + L-(-)carnitine
-
r
O-acetylcarnitine + CoASH
-
Rattus norvegicus acetyl-CoA + carnitine
-
r
O-acetylcarnitine + CoASH
-
Sus scrofa acetyl-CoA + carnitine
-
r
O-acetylcarnitine + CoASH
-
Columba sp. acetyl-CoA + carnitine
-
r

Subunits

Subunits Comment Organism
dimer 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
monomer
-
Rattus norvegicus
monomer 1 * 75000, SDS-PAGE Columba sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40
-
Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 50 enzyme retains total activity when kept at 37°C for 30 min, rapid loss of activity above 37°C, loses half of the activity at 40°C for 30 min, but is completely inactivated when kept at 44°C for 30 min or 50°C for 5 min, 75% loss of activity at 37°C for 8 h, only 15% loss when kept at 25°C for 8 h Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
DL-carnitine
-
Rattus norvegicus
8
-
palmityl-CoA substrate carnitine Rattus norvegicus
18.6
-
palmityl-CoA substrate acetyl-CoA Rattus norvegicus
920
-
acetylcarnitine substrate DL-carnitine Rattus norvegicus