Cloned (Comment) | Organism |
---|---|
for transfection of CHO-K1 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F174A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
I160A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
I162A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
I167A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
I170A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
L166A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
V173A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
V175A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
W163A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
Y169A | mutation of hydrophobic residue for measuring the remaining activity using acetyl-CoA and palmitoyl-CoA as acyl donors | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Mus musculus | |
Fe2+ | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0824 | - |
acetyl-CoA | apparent KM | Mus musculus | |
0.1282 | - |
1-O-hexadecyl-sn-glycero-3-phosphocholine | apparent KM | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Mus musculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
determined by SDS-PAGE and Western Blot analysis | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA | Mus musculus | lyso-PAF, lyso-platelet-activating factor | 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA | PAF, platelet-activating factor | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q3TFD2 | - |
- |
Purification (Comment) | Organism |
---|---|
LPCAT1 is partially purified from microsomes from mouse tissue using a DEAE-Sepharose column | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
lung | - |
Mus musculus | - |
spleen | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000017 | - |
brain, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000104 | Mus musculus |
0.000104 | - |
spleen, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000470 | Mus musculus |
0.00033 | - |
lung, Ca2+ independent lyso-PAF AT activity in the presence of EDTA, in the presence of Ca2+ 0.000452 | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA | lyso-PAF, lyso-platelet-activating factor | Mus musculus | 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoA | PAF, platelet-activating factor | ? | |
additional information | LPCAT1 recognizes a wide range of acyl-CoAs as substrates, ranging from acetyl-CoA to palmitoyl-CoA | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LPCAT1 | Ca2+ independent lyso-PAF acetyltransferase | Mus musculus |
lyso-PAF acetyltransferase | - |
Mus musculus |
lyso-PAF AT | - |
Mus musculus |
lysophosphatidylcholine acyltransferase 1 | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mus musculus |