Activating Compound | Comment | Organism | Structure |
---|---|---|---|
A23187 | - |
Homo sapiens |
General Stability | Organism |
---|---|
1-alkyl-sn-glycero-3-phosphocholine at low concentration and lysophosphatidylcholine stabilizes microsomal preparation | Homo sapiens |
dithiothreitol, 1 mM stabilizes microsomal preparation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine | irreversible inhibition dependent of both 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine and enzyme concentration | Homo sapiens | |
1-O-alkyl-sn-glycero-3-phosphocholine | inhibition at high concentrations due to its detergent effect | Homo sapiens | |
lysophosphatidylcholine | inhibition at high concentrations due to its detergent effect | Homo sapiens | |
phenylmethylsulfonyl fluoride | 30% inhibition at 50 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neutrophil | polymorphonuclear, ionophore A23187 stimulates | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
influence of phospholipids on specific activity at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 1-hexadecyl-2-lyso-sn-glycero-3-phosphocholine | 40% higher activity respect to the C18 analogue | Homo sapiens | CoA + 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
acetyl-CoA + 1-octadecyl-2-lyso-sn-glycero-3-phosphocholine | - |
Homo sapiens | CoA + 2-acetyl-1-octadecyl-sn-glycero-3-phosphocholine | - |
? | |
acetyl-CoA + acetyl-phosphatidylcholine | - |
Homo sapiens | ? | - |
? | |
acetyl-CoA + lyso-phosphatidylcholine | - |
Homo sapiens | CoA + 2-acetyl-1-acylphosphatidylcholine | - |
? | |
acetyl-CoA + lyso-phosphatidylethanolamine | - |
Homo sapiens | CoA + 2-acetyl-1-acyl-phosphatidylethanolamine | - |
? | |
acetyl-CoA + phosphatidylcholine | - |
Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
85% loss of activity after 2 h, 35% loss of activity after 2 h in the presence of DTT and 1-alkyl-2-lyso-sn-glycero-3-phosphate, microsomal preparation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |