Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.65 extracted from

  • Sfakianos, M.K.; Wilson, L.; Sakalian, M.; Falany, C.N.; Barnes, S.
    Conserved residues in the putative catalytic triad of human bile acid coenzyme A:amino acid N-acyltransferase (2002), J. Biol. Chem., 277, 47270-47275.
    View publication on PubMed
Search for more literature for 2.3.1.65

Cloned(Commentary)

Cloned (Comment) Organism
gene/full-length cDNA is cloned and expressed in Escherichia coli XL1-Blue or BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C235S mutant with lower enzyme activity Homo sapiens
C372A mutant with low enzyme activity Homo sapiens
D328A inactive enzyme Homo sapiens
H362A inactive enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Homo sapiens
N-ethylmaleimide 10 min, at 0.2 mM: 90% loss of activity, preincubation with cholyl-CoA before NEM-treatment protects Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choloyl-CoA + glycine Homo sapiens conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility CoA + glycocholate
-
 ?
choloyl-CoA + glycine Homo sapiens taurine can replace glycine, involved in conjugation of bile acids CoA + glycocholate
-
 ?
choloyl-CoA + taurine Homo sapiens conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility CoA + taurocholate
-
 ?
choloyl-CoA + taurine Homo sapiens taurine can replace glycine, involved in conjugation of bile acids CoA + taurocholate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
choloyl-CoA + glycine = CoA + glycocholate catalytic mechanism Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choloyl-CoA + 2-fluoro-beta-alanine 2-fluoro-beta-alanine is an excellent substrate like taurine Homo sapiens CoA + 2-fluoro-beta-alanylcholate
-
 ?
choloyl-CoA + beta-alanine beta-alanine is a poor substrate Homo sapiens CoA + beta-alanylcholate
-
 ?
choloyl-CoA + glycine
-
 Homo sapiens CoA + glycocholate
-
 ?
choloyl-CoA + glycine conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility Homo sapiens CoA + glycocholate
-
 ?
choloyl-CoA + glycine taurine can replace glycine, involved in conjugation of bile acids Homo sapiens CoA + glycocholate
-
 ?
choloyl-CoA + taurine taurine is an excellent substrate Homo sapiens CoA + taurocholate
-
 ?
choloyl-CoA + taurine conjugation of bile acids with glycine or taurine favors their excretion into bile and uptake from portal blood into the liver, it promotes absorption of fat and fat-soluble vitamins in the acidic environment of the small intestine by lowering the pKa of the bile acids and hence maintaining its solubility Homo sapiens CoA + taurocholate
-
 ?
choloyl-CoA + taurine taurine can replace glycine, involved in conjugation of bile acids Homo sapiens CoA + taurocholate
-
 ?
additional information essential catalytic triad consisting of Cys-235, His-362 and Asp-328 with Cys-235 serving as the probable nucleophile and thus the site of covalent attachment of the bile acid molecule Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
hBAT
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens