Cloned (Comment) | Organism |
---|---|
PCR-amplification, expression in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
agmatine | the presence of this substrate inhibits the enzyme reaction with putrescine | Arabidopsis thaliana | |
enterokinase | the removal of the thioredoxin domain by enterokinase decreases the enzyme activity but does not alter the relative amounts of products | Arabidopsis thaliana | |
additional information | putrescine or feruloyl-CoA do not inhibit the enzyme reaction with agmantine and p-coumaroyl-CoA | Arabidopsis thaliana | |
p-Coumaroyl-CoA | the presence of this substrate inhibits the enzyme reaction with feruloyl-CoA | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + agmatine | Arabidopsis thaliana | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | CoA + feruloyl agmatine | - |
? | |
feruloyl-CoA + putrescine | Arabidopsis thaliana | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | CoA + feruloylputrescine | - |
? | |
p-coumaroyl-CoA + agmatine | Arabidopsis thaliana | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | CoA + p-coumaroylagmatine | - |
? | |
p-coumaroyl-CoA + putrescine | Arabidopsis thaliana | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | CoA + p-coumaroylputrescine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
wild-type and mutants, infection of rosette leaves with the fungus Alternaria brassicicola strain MAFF237450 | - |
Purification (Comment) | Organism |
---|---|
frozen leaves are homogenized in 100 mM Tris buffer, pH 7.5, containing 10 mM 2-mercaptoethanol and 5 mM DTT, centrifugation, supernatant applied to Sephadex G-50 column, fluent used for enzyme assays, or recombinant Escherichia coli cells are harvested and resuspended in 100 mM Tris buffer, pH 7.5, containing 1 mM EDTA, 50 mM NaCl, 10% glycerol, lysed with lysozyme, sonicated, centrifuged, supernatant used for enzyme assays and SDS-PAGE | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | cut off rosette leaves infected with the fungus Alternaria brassicicola | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000003 | - |
with substrates p-coumaroyl-CoA + agmatine + putrescine 0.0018 nmol coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.000000085 | - |
with substrates feruloyl-CoA + putrescine 0.0051 nmol feruloylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.000000933 | - |
with substrates p-coumaroyl-CoA + putrescine 0.056 nmol p-coumaroylputrescine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.00002 | - |
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 1.2 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.00004 | - |
with substrates feruloyl-CoA + agmatine 2.4 nmol feruloylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.000112 | - |
with substrates p-coumaroyl-CoA + agmatine 6.7 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.000123 | - |
with substrates p-coumaroyl-CoA + agmatine + putrescine 7.4 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
0.000187 | - |
with substrates p-coumaroyl-CoA + feruloyl-CoA + agmatine 11.2 nmol p-coumaroylagmatine/mg/protein/h, recombinant enzyme, 30°C, pH 7.5 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + agmatine | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | Arabidopsis thaliana | CoA + feruloyl agmatine | - |
? | |
feruloyl-CoA + putrescine | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | Arabidopsis thaliana | CoA + feruloylputrescine | - |
? | |
p-coumaroyl-CoA + agmatine | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | Arabidopsis thaliana | CoA + p-coumaroylagmatine | - |
? | |
p-coumaroyl-CoA + putrescine | 30°C, pH 7.5, pathway analysis with stable isotope-labeled precursors | Arabidopsis thaliana | CoA + p-coumaroylputrescine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
agmatine coumaroyltransferase | - |
Arabidopsis thaliana |
At5g61160 protein | - |
Arabidopsis thaliana |
AtACT | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | upon infection with Alternaria brassicicola enzyme activity in leaves increases markedly from near zero to almost 20 nmol/mg protein/h within 24 h coinciding with an increase in gene transcripts and in the synthesis products, that accumulate in the area of the lesion, slow decrease beyond 24 h | up |
General Information | Comment | Organism |
---|---|---|
metabolism | last step in the synthesis of hydroxycinnamic acid amides in plants (anti-pathogenic metabolites), in Arabidopsis thaliana infected with Alternaria brassicicola p-coumaroylagmatine, feruloylagmatine, p-coumaroylputrescine, and feruloylputrescine are identified as anti-fungal agents in vitro and in vivo | Arabidopsis thaliana |