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Literature summary for 2.3.1.64 extracted from

  • Burhenne, K.; Kristensen, B.K.; Rasmussen, S.K.
    A new class of N-hydroxycinnamoyltransferases. Purification, cloning, and expression of barley agmatine coumaroyltransferase (EC 2.3.1.64) (2003), J. Biol. Chem., 278, 13919-13927.
    View publication on PubMed
Search for more literature for 2.3.1.64

Activating Compound

Activating Compound Comment Organism Structure
1-mercapto-2,3-propanediol 1 mM is required for optimal activity Hordeum vulgare
2-mercaptoethanol 1 mM is required for optimal activity Hordeum vulgare

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 10 mM, 85% inhibition Hordeum vulgare
dithiothreitol 30% inhibition Hordeum vulgare
MnCl2 10 mM, 29% inhibition Hordeum vulgare
ZnSO4 10 mM, 99% inhibition Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 4-coumaroyl-CoA isoenzyme 1 Hordeum vulgare
0.0016
-
 4-coumaroyl-CoA isoenzyme 3 Hordeum vulgare
0.0017
-
 feruloyl-CoA isoenzyme 3 Hordeum vulgare
0.0021
-
 4-coumaroyl-CoA isoenzyme 2 Hordeum vulgare
0.0021
-
 4-coumaroyl-CoA recombinant enzyme Hordeum vulgare
0.0041
-
 feruloyl-CoA isoenzyme 2 Hordeum vulgare
0.0043
-
 caffeoyl-CoA isoenzyme 2 Hordeum vulgare
0.0043
-
 caffeoyl-CoA recombinant enzyme Hordeum vulgare
0.0052
-
 agmatine isoenzyme 1 Hordeum vulgare
0.0052
-
 agmatine isoenzyme 3 Hordeum vulgare
0.0053
-
 caffeoyl-CoA isoenzyme 3 Hordeum vulgare
0.0058
-
 caffeoyl-CoA isoenzyme 1 Hordeum vulgare
0.0061
-
 feruloyl-CoA isoenzyme 1 Hordeum vulgare
0.0065
-
 agmatine isoenzyme 2 Hordeum vulgare
0.0066
-
 feruloyl-CoA recombinant enzyme Hordeum vulgare
0.0081
-
 agmatine recombinant enzyme Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
-
 Hordeum vulgare
48000
-
 1 * 48000, SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare A9ZPJ6 isoenzymes 1-3
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.78
-
-
 Hordeum vulgare
11.34
-
 recombinant enzyme Hordeum vulgare

Storage Stability

Storage Stability Organism
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + agmatine
-
 Hordeum vulgare CoA + 4-coumaroylagmatine
-
 ?
caffeoyl-CoA + agmatine
-
 Hordeum vulgare CoA + caffeoylagmatine
-
 ?
feruloyl-CoA + agmatine
-
 Hordeum vulgare CoA + feruloyl agmatine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48000, SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Hordeum vulgare

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
 Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
6.9 8.1 half-maximal activity at pH 6.9 and pH 8.1 Hordeum vulgare