Literature summary for 2.3.1.61 extracted from

Guo, H.; Madzak, C.; Du, G.; Zhou, J.
Mutagenesis of conserved active site residues of dihydrolipoamide succinyltransferase enhances the accumulation of alpha-ketoglutarate in Yarrowia lipolytica (2016), Appl. Microbiol. Biotechnol., 100, 649-659 .

Search for more literature for 2.3.1.61

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Yarrowia lipolytica

Protein Variants

Protein Variants	Comment	Organism
D423A	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
D423DEL	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
D423E	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
D423G	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
D423S	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
H419A	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
H419D	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
H419del	inactive	Yarrowia lipolytica
H419G	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica
H419S	the mutant shows reduced activity compared to the wild type enzyme	Yarrowia lipolytica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	DL-dihydrolipoamide	mutant enzyme H419S, at pH 8.0 and 30°C	Yarrowia lipolytica
0.025	-	DL-dihydrolipoamide	mutant enzyme H419DEL, at pH 8.0 and 30°C	Yarrowia lipolytica
0.026	-	DL-dihydrolipoamide	mutant enzyme H419A, at pH 8.0 and 30°C	Yarrowia lipolytica
0.026	-	DL-dihydrolipoamide	mutant enzyme H419G, at pH 8.0 and 30°C	Yarrowia lipolytica
0.0274	-	DL-dihydrolipoamide	wild type enzyme, at pH 8.0 and 30°C	Yarrowia lipolytica
0.03	-	DL-dihydrolipoamide	mutant enzyme H419D, at pH 8.0 and 30°C	Yarrowia lipolytica
0.043	-	DL-dihydrolipoamide	mutant enzyme D423S, at pH 8.0 and 30°C	Yarrowia lipolytica
0.052	-	DL-dihydrolipoamide	mutant enzyme D423G, at pH 8.0 and 30°C	Yarrowia lipolytica
0.062	-	DL-dihydrolipoamide	mutant enzyme D423A, at pH 8.0 and 30°C	Yarrowia lipolytica
0.062	-	DL-dihydrolipoamide	mutant enzyme D423DEL, at pH 8.0 and 30°C	Yarrowia lipolytica
0.09	-	DL-dihydrolipoamide	mutant enzyme D423E, at pH 8.0 and 30°C	Yarrowia lipolytica

Organism

Organism	UniProt	Comment	Textmining
Yarrowia lipolytica	-	-	-
Yarrowia lipolytica WSH-Z06	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-CoA + DL-dihydrolipoamide	-	Yarrowia lipolytica	CoA + S-succinyldihydrolipoamide	-	?
succinyl-CoA + DL-dihydrolipoamide	-	Yarrowia lipolytica WSH-Z06	CoA + S-succinyldihydrolipoamide	-	?

Synonyms

Synonyms	Comment	Organism
dihydrolipoamide succinyltransferase	-	Yarrowia lipolytica
DLST	-	Yarrowia lipolytica
KGD2	-	Yarrowia lipolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3	-	DL-dihydrolipoamide	mutant enzyme D423G, at pH 8.0 and 30°C	Yarrowia lipolytica
3	-	DL-dihydrolipoamide	mutant enzyme H419G, at pH 8.0 and 30°C	Yarrowia lipolytica
6	-	DL-dihydrolipoamide	mutant enzyme H419D, at pH 8.0 and 30°C	Yarrowia lipolytica
7	-	DL-dihydrolipoamide	mutant enzyme D423A, at pH 8.0 and 30°C	Yarrowia lipolytica
7	-	DL-dihydrolipoamide	mutant enzyme D423DEL, at pH 8.0 and 30°C	Yarrowia lipolytica
7	-	DL-dihydrolipoamide	mutant enzyme H419A, at pH 8.0 and 30°C	Yarrowia lipolytica
11	-	DL-dihydrolipoamide	mutant enzyme H419S, at pH 8.0 and 30°C	Yarrowia lipolytica
18	-	DL-dihydrolipoamide	mutant enzyme D423S, at pH 8.0 and 30°C	Yarrowia lipolytica
165	-	DL-dihydrolipoamide	mutant enzyme D423E, at pH 8.0 and 30°C	Yarrowia lipolytica
186	-	DL-dihydrolipoamide	wild type enzyme, at pH 8.0 and 30°C	Yarrowia lipolytica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
7000	-	DL-dihydrolipoamide	wild type enzyme, at pH 8.0 and 30°C	Yarrowia lipolytica

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Release 2023.1 (January 2023)