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Literature summary for 2.3.1.60 extracted from

  • Xu, Z.; Stogios, P.J.; Quaile, A.T.; Forsberg, K.J.; Patel, S.; Skarina, T.; Houliston, S.; Arrowsmith, C.; Dantas, G.; Savchenko, A.
    Structural and functional survey of environmental aminoglycoside acetyltransferases reveals functionality of resistance enzymes (2017), ACS Infect. Dis., 3, 653-665 .
    View publication on PubMed
Search for more literature for 2.3.1.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-Gold cells uncultured bacterium
expressed in Escherichia coli BL21(DE3)-Gold cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, sitting drop vapor diffusion method, using 40% (w/v) PEG 300 and phosphate-citrate (pH 5.2) Pseudomonas aeruginosa
in complex with CoA, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M magnesium formate uncultured bacterium

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q51407
-
-
uncultured bacterium
-
-
-
uncultured bacterium A0A059WV44
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration uncultured bacterium
Ni-NTA column chromatography and Superdex S200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + gentamicin
-
 uncultured bacterium CoA + N3-acetylgentamicin
-
 ?
acetyl-CoA + gentamicin
-
 Pseudomonas aeruginosa CoA + N3-acetylgentamicin
-
 ?
acetyl-CoA + kanamycin
-
 uncultured bacterium CoA + N3''-acetylkanamycin
-
 ?
acetyl-CoA + kanamycin
-
 Pseudomonas aeruginosa CoA + N3''-acetylkanamycin
-
 ?
acetyl-CoA + sisomicin
-
 uncultured bacterium CoA + N3-acetylsisomicin
-
 ?
acetyl-CoA + sisomicin
-
 Pseudomonas aeruginosa CoA + N3-acetylsisomicin
-
 ?
acetyl-CoA + tobramycin
-
 uncultured bacterium CoA + N3-acetyltobramycin
-
 ?
acetyl-CoA + tobramycin
-
 Pseudomonas aeruginosa CoA + N3-acetyltobramycin
-
 ?

Synonyms

Synonyms Comment Organism
AAC
-
 uncultured bacterium
AAC
-
 Pseudomonas aeruginosa
AAC(3)-Ib
-
 Pseudomonas aeruginosa
aminoglycoside acetyltransferase
-
 uncultured bacterium
aminoglycoside acetyltransferase
-
 Pseudomonas aeruginosa
aminoglycoside N-acetyltransferase
-
 uncultured bacterium
aminoglycoside N-acetyltransferase
-
 Pseudomonas aeruginosa
meta-AAC0005
-
 uncultured bacterium
meta-AAC0007
-
 uncultured bacterium
meta-AAC0012
-
 uncultured bacterium

General Information

General Information Comment Organism
physiological function the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin uncultured bacterium
physiological function the enzyme confers high-level heterologous resistance to the aminoglycosides gentamicin C, sisomicin, and tobramycin Pseudomonas aeruginosa