Any feedback?
Literature summary for 2.3.1.60 extracted from
- Purification and properties of gentamicin acetyltransferase I (1976), Biochemistry, 15, 125-131.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | N-methylmaleimide and DTT have no effect on activity | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| lyophilization, stable to | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| gentamicin | - |
Escherichia coli |  |
| gentamicin C1a | substrate inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | 0.0005 | gentamicin C1a | - |
Escherichia coli | |
| 0.0039 | 0.0048 | acetyl-CoA | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
4 * 17000, SDS-PAGE | Escherichia coli |
| 63000 | - |
equilibrium sedimentation centrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
containing R-factor JR88 | - |
| Escherichia coli C600 | - |
containing R-factor JR88 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, lyophilized, up to 1 month | Escherichia coli |
| frozen, redissolved ammonium sulfate precipitate, at least 1 month | Escherichia coli |
| refrigerated, lyophilized, up to 1 month | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + gentamicin C1a | - |
Escherichia coli | CoA + N3'-acetylgentamicin C1a | - |
? | |
| acetyl-CoA + gentamicin C1a | - |
Escherichia coli C600 | CoA + N3'-acetylgentamicin C1a | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 17000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.57 | - |
N3'-acetylgentamicin C1a | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | 8 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
irreversible inactivation | Escherichia coli |
| 4.5 | - |
no activity, restorable by neutralization | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
gentamicin | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
