Literature summary for 2.3.1.6 extracted from

Wu, D.; Hersh, L.B.
Identification of an active site arginine in rat choline acetyltransferase by alanine scanning mutagenesis (1995), J. Biol. Chem., 270, 29111-29116.

Search for more literature for 2.3.1.6

Protein Variants

Protein Variants	Comment	Organism
R452A	kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues	Rattus norvegicus
R453A	kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues	Rattus norvegicus
R458A	kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues	Rattus norvegicus
R463A	kinetic as well chemical modification studies have implicated the presence of an active site arginine in enzyme, whose function is to stabilize coenzyme binding, conserved arginines are converted to identify these residues	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00025	-	CoA	-	Rattus norvegicus
0.0004	-	Acetylcholine	-	Rattus norvegicus
0.0004	-	CoA	enzyme mutant R458A	Rattus norvegicus
0.001	-	CoA	enzyme mutant R453A	Rattus norvegicus
0.0011	-	Acetylcholine	enzyme mutant R463A	Rattus norvegicus
0.0015	-	Acetylcholine	enzyme mutant R453A	Rattus norvegicus
0.0016	-	CoA	enzyme mutant R463A	Rattus norvegicus
0.002	-	Acetylcholine	enzyme mutant R458A	Rattus norvegicus
0.0092	-	Acetylcholine	enzyme mutant R452A	Rattus norvegicus
0.0154	-	CoA	enzyme mutant R452A	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + choline	-	Rattus norvegicus	acetylcholine + CoA	-	?

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Release 2023.1 (January 2023)