Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
spermine | at pH 7.5 and 30°C | Enterococcus faecalis | |
0.295 | - |
spermine | at pH 7.5 and 30°C | Staphylococcus aureus | |
0.769 | - |
spermidine | at pH 7.5 and 30°C | Enterococcus faecalis | |
1.33 | - |
spermidine | at pH 7.5 and 30°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + norspermidine | Staphylococcus aureus | - |
CoA + N1-acetylnorspermidine | - |
? | |
acetyl-CoA + norspermidine | Staphylococcus aureus USA300 | - |
CoA + N1-acetylnorspermidine | - |
? | |
acetyl-CoA + spermidine | Staphylococcus aureus | - |
CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermidine | Staphylococcus aureus USA300 | - |
CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermine | Staphylococcus aureus | preferred substrate | CoA + N1-acetylspermine | - |
? | |
acetyl-CoA + spermine | Staphylococcus aureus USA300 | preferred substrate | CoA + N1-acetylspermine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Staphylococcus aureus | A0A0H2XGJ0 | - |
- |
Staphylococcus aureus USA300 | A0A0H2XGJ0 | - |
- |
Purification (Comment) | Organism |
---|---|
Hi-Trap column chromatography | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N1-acetylspermine | - |
Enterococcus faecalis | CoA + N1,N12-diacetylspermine | - |
? | |
acetyl-CoA + norspermidine | - |
Enterococcus faecalis | CoA + N1-acetylnorspermidine | - |
? | |
acetyl-CoA + norspermidine | - |
Staphylococcus aureus | CoA + N1-acetylnorspermidine | - |
? | |
acetyl-CoA + norspermidine | - |
Staphylococcus aureus USA300 | CoA + N1-acetylnorspermidine | - |
? | |
acetyl-CoA + spermidine | - |
Enterococcus faecalis | CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermidine | - |
Staphylococcus aureus | CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermidine | - |
Staphylococcus aureus USA300 | CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermine | preferred substrate | Enterococcus faecalis | CoA + N1-acetylspermine | - |
? | |
acetyl-CoA + spermine | preferred substrate | Staphylococcus aureus | CoA + N1-acetylspermine | - |
? | |
acetyl-CoA + spermine | preferred substrate | Staphylococcus aureus USA300 | CoA + N1-acetylspermine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bltD | - |
Enterococcus faecalis |
SpeG | - |
Staphylococcus aureus |
spermidine/spermine N-acetyltransferase | - |
Enterococcus faecalis |
spermidine/spermine N-acetyltransferase | - |
Staphylococcus aureus |
SSAT | - |
Enterococcus faecalis |
SSAT | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.58 | - |
spermidine | at pH 7.5 and 30°C | Enterococcus faecalis | |
2.48 | - |
spermidine | at pH 7.5 and 30°C | Staphylococcus aureus | |
3.52 | - |
spermine | at pH 7.5 and 30°C | Staphylococcus aureus | |
9.21 | - |
spermine | at pH 7.5 and 30°C | Enterococcus faecalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
spermidine | at pH 7.5 and 30°C | Staphylococcus aureus | |
2 | - |
spermidine | at pH 7.5 and 30°C | Enterococcus faecalis | |
12 | - |
spermine | at pH 7.5 and 30°C | Staphylococcus aureus | |
200 | - |
spermine | at pH 7.5 and 30°C | Enterococcus faecalis |