Literature summary for 2.3.1.57 extracted from

Sugiyama, S.; Ishikawa, S.; Tomitori, H.; Niiyama, M.; Hirose, M.; Miyazaki, Y.; Higashi, K.; Murata, M.; Adachi, H.; Takano, K.; Murakami, S.; Inoue, T.; Mori, Y.; Kashiwagi, K.; Igarashi, K.; Matsumura, H.
Molecular mechanism underlying promiscuous polyamine recognition by spermidine acetyltransferase (2016), Int. J. Biochem. Cell Biol., 76, 87-97 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with spermidine and CoA, sitting drop vapor diffusion method, using 0.1 M Ca-acetate, 0.05 M Na-cacodylate pH 6.5, and 9% (w/v) PEG8000	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
E33Q	the mutant shows greatly reduced enzyme activity compared to the wild type enzyme	Escherichia coli
E34Q	inactive	Escherichia coli
E37Q	inactive	Escherichia coli
E41Q	inactive	Escherichia coli
E55Q	the mutant shows greatly reduced enzyme activity compared to the wild type enzyme	Escherichia coli
E71Q	inactive	Escherichia coli
E74Q	the mutant shows greatly reduced enzyme activity compared to the wild type enzyme	Escherichia coli
E83Q	the mutant shows greatly reduced enzyme activity compared to the wild type enzyme	Escherichia coli
O85E	the mutant shows greatly reduced enzyme activity compared to the wild type enzyme	Escherichia coli
Q53A	the mutant shows greatly reduced enzyme activity compared to the wild type enzyme	Escherichia coli
R56A	inactive	Escherichia coli
W31A	inactive	Escherichia coli
Y133F	the mutant shows increased enzyme activity compared to the wild type enzyme	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + spermidine	Escherichia coli	-	CoA + N1-acetylspermidine	-	?
acetyl-CoA + spermidine	Escherichia coli LY180	-	CoA + N1-acetylspermidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	A0A0M3KKU5	-	-
Escherichia coli LY180	A0A0M3KKU5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + spermidine	-	Escherichia coli	CoA + N1-acetylspermidine	-	?
acetyl-CoA + spermidine	-	Escherichia coli LY180	CoA + N1-acetylspermidine	-	?

Subunits

Subunits	Comment	Organism
dodecamer	X-ray crystallography, the dodecamer is organized as a hexamer of dimers	Escherichia coli

Synonyms

Synonyms	Comment	Organism
SAT	-	Escherichia coli
spermidine acetyltransferase	-	Escherichia coli
spermidine/spermine N1-acetyltransferase	-	Escherichia coli
SSAT	-	Escherichia coli

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Release 2023.1 (January 2023)