Literature summary for 2.3.1.57 extracted from

Filippova, E.V.; Weigand, S.; Kiryukhina, O.; Wolfe, A.J.; Anderson, W.F.
Analysis of crystalline and solution states of ligand-free spermidine N-acetyltransferase (SpeG) from Escherichia coli (2019), Acta Crystallogr. Sect. D, 75, 545-553 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M ammonium phosphate monobasic, 0.1 M Tris, 50% (w/v) 2-methyl-2,4-pentanediol, pH 8.5	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26300	-	calculated from amino acid sequence	Escherichia coli
270000	-	SEC-MALS analysis	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + spermidine	Escherichia coli	-	CoA + N1-acetylspermidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A951	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex S200 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + spermidine	-	Escherichia coli	CoA + N1-acetylspermidine	-	?

Subunits

Subunits	Comment	Organism
dodecamer	12 * 21900, X-ray crystallography	Escherichia coli

Synonyms

Synonyms	Comment	Organism
SpeG	-	Escherichia coli
spermidine N-acetyltransferase	-	Escherichia coli

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Release 2023.1 (January 2023)