Any feedback?
Literature summary for 2.3.1.57 extracted from
- Metabolism of triethylenetetramine and 1,12-diamino-3,6,9-triazadodecane by the spermidine/spermine-N1-acetyltransferase and thialysine acetyltransferase (2013), Drug Metab. Dispos., 41, 30-32.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | knockdown of enzyme SSAT1 in Hep-G2 cells and in primary hepatocytes | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
triethylenetetramine | pH and temperature not specified in the publication | Homo sapiens |  |
| 0.106 | - |
1,12-diamino-3,6,9-triazadodecane | pH and temperature not specified in the publication | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 acetyl-CoA + 2 spermine | Homo sapiens | - |
3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
| 3 acetyl-CoA + 2 spermine | Mus musculus | - |
3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
| 3 acetyl-CoA + spermidine | Homo sapiens | - |
3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
| 3 acetyl-CoA + spermidine | Mus musculus | - |
3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
| acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane | Homo sapiens | an extremely poor substrate of human recombinant SSAT2, that is metabolized by SSAT1 in Hep-G2 cells and in wild-type primary hepatocytes | ? | - |
? | |
| acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane | Mus musculus | SSAT1 is the main acetylator of 1,12-diamino-3,6,9-triazadodecane compared to SSAT2 | ? | - |
? | |
| acetyl-CoA + triethylenetetramine | Homo sapiens | SSAT2 is the main acetylator of TETA compared to SSAT1 | ? | - |
? | |
| acetyl-CoA + triethylenetetramine | Mus musculus | SSAT2 is the main acetylator of TETA compared to SSAT1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P21673 | - |
- |
| Mus musculus | P48026 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
| hepatocyte | primary | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3 acetyl-CoA + 2 spermine | - |
Homo sapiens | 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
| 3 acetyl-CoA + 2 spermine | - |
Mus musculus | 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
| 3 acetyl-CoA + spermidine | - |
Homo sapiens | 3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
| 3 acetyl-CoA + spermidine | - |
Mus musculus | 3 CoA + N1,N4,N8-triacetylspermidine | - |
? | |
| acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane | an extremely poor substrate of human recombinant SSAT2, that is metabolized by SSAT1 in Hep-G2 cells and in wild-type primary hepatocytes | Homo sapiens | ? | - |
? | |
| acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane | SSAT1 is the main acetylator of 1,12-diamino-3,6,9-triazadodecane compared to SSAT2 | Homo sapiens | ? | - |
? | |
| acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane | SSAT1 is the main acetylator of 1,12-diamino-3,6,9-triazadodecane compared to SSAT2 | Mus musculus | ? | - |
? | |
| acetyl-CoA + triethylenetetramine | SSAT2 is the main acetylator of TETA compared to SSAT1 | Homo sapiens | ? | - |
? | |
| acetyl-CoA + triethylenetetramine | SSAT2 is the main acetylator of TETA compared to SSAT1 | Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| spermidine/spermine-N1-acetyltransferase | - |
Homo sapiens |
| spermidine/spermine-N1-acetyltransferase | - |
Mus musculus |
| SSAT1 | - |
Homo sapiens |
| SSAT1 | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | acetylation of triethylenetetramine is increased in SSAT1-overexpressing mice compared with wild-type mice, but SSAT1-deficient mice metabolize TETA at the same rate as the wild-type mice, due to the activity of thialysine acetyltransferase (SSAT2) | Mus musculus |
| malfunction | SSAT1 knockdown leads to a dramatic reduction of N1-acetyylspermidine and N1-acetylspermine pools. Metabolism of 1,12-diamino-3,6,9-triazadodecane to N1-acetyl-1,12-diamino-3,6,9-triazadodecane is reduced with SSAT1 but not with SSAT2 shRNA. No metabolism of 1,12-diamino-3,6,9-triazadodecane detectable in SSAT1-KO cells. In wild-type cells, SSAT2 knockdown does not reduce the metabolism of 1,12-diamino-3,6,9-triazadodecane to N1-AcSpmTrien. In fact it leads to the induction of SSAT1 activity and increased metabolism of 1,12-diamino-3,6,9-triazadodecane to N1-acetyl-1,12-diamino-3,6,9-triazadodecane | Homo sapiens |
| metabolism | acetylation of triethylenetetramine is increased in SSAT1-overexpressing mice compared with wild-type mice, but SSAT1-deficient mice metabolize TETA at the same rate as the wild-type mice, due to the activity of thialysine acetyltransferase (SSAT2) | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
