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Literature summary for 2.3.1.57 extracted from

  • Zhang, Y.; Zhou, J.; Chang, M.; Bai, L.; Shan, J.; Yao, C.; Jiang, R.; Guo, L.; Zhang, R.; Wu, J.; Li, Y.
    Characterization of and functional evidence for Ste27 of Streptomyces sp. 139 as a novel spermine/spermidine acetyltransferase (2012), Biochem. J., 443, 727-734.
    View publication on PubMed
Search for more literature for 2.3.1.57

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure. Both spermidine and spermine should bind at unique position with high specificity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 acetyl-CoA pH 8.0, 30°C Streptomyces sp.
0.065
-
 spermidine pH 8.0, 30°C Streptomyces sp.
0.215
-
 D-glucosamine 6-phosphate pH 8.0, 30°C Streptomyces sp.
0.338
-
 spermine pH 8.0, 30°C Streptomyces sp.
0.859
-
 poly-L-lysine pH 8.0, 30°C Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. D2CFQ9 putative
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 acetyl-CoA + spermidine spermidine is preferred over spermine Streptomyces sp. 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?
acetyl-CoA + D-glucosamine 6-phosphate
-
 Streptomyces sp. CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
acetyl-CoA + poly-L-lysine
-
 Streptomyces sp. CoA + acetyl-poly-L-lysine
-
 ?
acetyl-CoA + spermine spermidine is preferred over spermine Streptomyces sp. CoA + N1-acetylspermine
-
 ?
additional information no substrates: glycine, glutamate, aspartate, leucine, alanine or arginine Streptomyces sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
Ste27
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15
-
 about 50% of maximum activity Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.858
-
 acetyl-CoA pH 8.0, 30°C Streptomyces sp.
0.922
-
 spermidine pH 8.0, 30°C Streptomyces sp.
1.358
-
 D-glucosamine 6-phosphate pH 8.0, 30°C Streptomyces sp.
1.86
-
 spermine pH 8.0, 30°C Streptomyces sp.
3.953
-
 poly-L-lysine pH 8.0, 30°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 about 50% of maximum activity Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.76
-
 spermidine pH 8.0, 30°C Streptomyces sp.
4.6
-
 poly-L-lysine pH 8.0, 30°C Streptomyces sp.
8.63
-
 spermine pH 8.0, 30°C Streptomyces sp.
13.17
-
 acetyl-CoA pH 8.0, 30°C Streptomyces sp.
24
-
 D-glucosamine 6-phosphate pH 8.0, 30°C Streptomyces sp.