Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N1,N11-bis-(ethyl)-norspermine | induces mRNA and activity of SSAT protein, translation proceeded by competition of a predicted RNA-binding protein | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, FLAG-tagged SSAT protein and SSAT protein fused to Renilla luciferase | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
cloned from human breast cancer cDNA library | - |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | transient expression in | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
regulation of SSAT expression, de novo RNA and protein synthesis analyzed, two regions of the SSAT protein coding sequence involved in translational repression of SSAT in the absence of elevated polyamine levels, RNA-binding protein possibly interacting with stem-loop structures to block translation can be displaced by the polyamine analogue N1,N11-bis-(ethyl)-norspermine (DENSPM), allowing translation to proceed, 5' and 3' polyamine-responsive regions interact with each other in vivo, changes in either region result in translation in the absence of the polyamine analogue N1,N11-bis-(ethyl)-norspermine | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | polyamines regulate SSAT mRNA translational efficiency by inhibiting a repressor protein from binding to regions of the coding sequence of the SSAT transcript | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
spermidine/spermine N1-acetyltransferase | - |
Homo sapiens |
SSAT | - |
Homo sapiens |