Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.57 extracted from

  • Butcher, N.J.; Broadhurst, G.M.; Minchin, R.F.
    Polyamine-dependent regulation of spermidine-spermine N1-acetyltransferase mRNA translation (2007), J. Biol. Chem., 282, 28530-28539.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N1,N11-bis-(ethyl)-norspermine induces mRNA and activity of SSAT protein, translation proceeded by competition of a predicted RNA-binding protein Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, FLAG-tagged SSAT protein and SSAT protein fused to Renilla luciferase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
cloned from human breast cancer cDNA library
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell transient expression in Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
regulation of SSAT expression, de novo RNA and protein synthesis analyzed, two regions of the SSAT protein coding sequence involved in translational repression of SSAT in the absence of elevated polyamine levels, RNA-binding protein possibly interacting with stem-loop structures to block translation can be displaced by the polyamine analogue N1,N11-bis-(ethyl)-norspermine (DENSPM), allowing translation to proceed, 5' and 3' polyamine-responsive regions interact with each other in vivo, changes in either region result in translation in the absence of the polyamine analogue N1,N11-bis-(ethyl)-norspermine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polyamines regulate SSAT mRNA translational efficiency by inhibiting a repressor protein from binding to regions of the coding sequence of the SSAT transcript Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
spermidine/spermine N1-acetyltransferase
-
Homo sapiens
SSAT
-
Homo sapiens