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Literature summary for 2.3.1.57 extracted from

  • McCloskey, D.E.; Pegg, A.E.
    Properties of the spermidine/spermine N1-acetyltransferase mutant L156F that decreases cellular sensitivity to the polyamine analogue N1,N11-bis(ethyl)norspermine (2003), J. Biol. Chem., 278, 13881-13887.
    View publication on PubMed
Search for more literature for 2.3.1.57

Activating Compound

Activating Compound Comment Organism Structure
N1,N11-bis(ethyl)norspermine polyamine analogue, about 300fold activation of wild-type enzyme, 4fold activation of mutant L156F, the compound highly stabilizes the wild-type enzyme preventing it from degradation, while the effect on degradation of the mutant enzyme is smaller Cricetulus griseus

Cloned(Commentary)

Cloned (Comment) Organism
expression of the L156F mutant enzyme in C55.7Res cells, in vitro transcription and translation of His-tagged wild-type and mutant L156F enzymes Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
L156F mutant enzyme shows about 20fold reduced activity and over 70fold reduced activation by the polyamine analogue N1,N11-bis(ethyl)norspermine compared to the wild-type enzyme, expression of the mutant enzymes decreases cellular sensitivity to N1,N11-bis(ethyl)norspermine Cricetulus griseus

General Stability

General Stability Organism
N1,N11-bis(ethyl)norspermine completely stabilizes the wild-type enzyme, which is degraded within 90 min in absence of N1,N11-bis(ethyl)norspermine, half-life: 22 min, half-life in presence of N1,N11-bis(ethyl)norspermine: 144 h, while for the mutant L156F half-life is 105 min and 154 min, respectively Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 spermine pH 7.8, 30°C, recombinant wild-type enzyme Cricetulus griseus
0.037
-
 spermine pH 7.8, 30°C, recombinant mutant L156F enzyme Cricetulus griseus
0.056
-
 spermidine pH 7.8, 30°C, recombinant wild-type enzyme Cricetulus griseus
0.3
-
 spermidine pH 7.8, 30°C, recombinant mutant L156F enzyme Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant L156F enzymes from in vitro transcription and translation Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cricetulus griseus
1.5
-
 purified recombinant mutant L156F Cricetulus griseus
26
-
 purified recombinant wild-type enzyme Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + spermidine
-
 Cricetulus griseus CoA + N1-acetyl-spermidine
-
 ?
acetyl-CoA + spermine
-
 Cricetulus griseus CoA + N1-acetyl-spermine
-
 ?

Synonyms

Synonyms Comment Organism
spermidine/spermine N1-acetyltransferase
-
 Cricetulus griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cricetulus griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.63
-
 spermidine pH 7.8, 30°C, recombinant mutant L156F enzyme Cricetulus griseus
0.63
-
 spermine pH 7.8, 30°C, recombinant mutant L156F enzyme Cricetulus griseus
5
-
 spermine pH 7.8, 30°C, recombinant wild-type enzyme Cricetulus griseus
8.7
-
 spermidine pH 7.8, 30°C, recombinant wild-type enzyme Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Cricetulus griseus