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Literature summary for 2.3.1.56 extracted from

  • Jones, R.F.; Land, S.J.; King, C.M.
    Recombinant rat and hamster N-acetyltransferases-1 and -2: relative rates of N-acetylation of arylamines and N,O-acyl transfer with arylhydroxamic acids (1996), Carcinogenesis, 17, 1729-1733.
    View publication on PubMed
Search for more literature for 2.3.1.56

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoform NAT1 in Escherichia coli Rattus norvegicus
expression of isoform NAT2 in Escherichia coli Rattus norvegicus
expression of isoforms NAT1 in Escherichia coli Mesocricetus auratus
expression of isoforms NAT2 in Escherichia coli Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00064
-
 4-aminoazobenzene
-
 Rattus norvegicus
0.00089
-
 4-aminoazobenzene
-
 Mesocricetus auratus
0.005
-
 N-Hydroxy-4-acetylaminobiphenyl recombinant enzyme Rattus norvegicus
0.0068
-
 N-Hydroxy-4-acetylaminobiphenyl recombinant enzyme Mesocricetus auratus
0.062
-
 4-aminoazobenzene
-
 Mesocricetus auratus
0.134
-
 N-Hydroxy-4-acetylaminobiphenyl recombinant enzyme Mesocricetus auratus
0.138
-
 4-aminoazobenzene
-
 Rattus norvegicus
0.356
-
 N-Hydroxy-4-acetylaminobiphenyl recombinant enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, isoform NAT2, SDS-PAGE Mesocricetus auratus
31000
-
 x * 31000, SDS-PAGE Rattus norvegicus
31000
-
 x * 31000, SDS-PAGE Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus P50292 isoform NAT1
-
Mesocricetus auratus P50293 isoform NAT2
-
Rattus norvegicus P50297 isoform NAT1
-
Rattus norvegicus P50298 isoform NAT2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
 recombinant enzyme, N-acetylation of 4-aminoazobenzene Rattus norvegicus
0.036
-
 recombinant enzyme, N-acetylation of 4-aminoazobenzene Mesocricetus auratus
0.084
-
 recombinant enzyme, N-acetylation of 4-aminoazobenzene Mesocricetus auratus
0.097
-
 recombinant enzyme, N-acetylation of 4-aminoazobenzene Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-hydroxy-4-acetylaminobiphenyl + 4-aminoazobenzene
-
 Rattus norvegicus N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminoazobenzene
-
 ?
N-hydroxy-4-acetylaminobiphenyl + 4-aminoazobenzene
-
 Mesocricetus auratus N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminoazobenzene
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Rattus norvegicus
? x * 31000, SDS-PAGE Mesocricetus auratus
? x * 29000, isoform NAT2, SDS-PAGE Mesocricetus auratus